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Research paper | Open Access

BSA-seq-based identification of a major additive plant height QTL with an effect equivalent to that of Semi-dwarf 1 in a large rice F2 population

Bo Zhanga, Feixiang Qia, Gang Hua, Yikai Yanga, Li Zhanga, Jianghu Menga, Zhongmin Hana, Xiangchun Zhoua, Haiyang Liua,b, Mohammed Ayaada,c, Yongzhong Xinga( )
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434100, Hubei, China
Plant Research Department, Nuclear Research Center, Atomic Energy Authority, Abo-Zaabal 13759, Egypt
Show Author Information

Abstract

Bulked-segregant analysis is a time- and cost-saving strategy for identifying major quantitative trait loci (QTL) in a mapping population. Bulked-segregant analysis combined with whole-genome sequencing (BSA-seq) was performed to rapidly identify QTL for heading date, plant height, and panicle length in a large F2 population derived from two landraces: Chuan 7 (C7) and Haoboka (HBK). Twenty plants with extremely low or high phenotypic values for the target traits were selected from an F2 population of 940 plants to construct low- and high-value bulks. Three pairs of bulks for the three traits were constructed, resulting in six DNA pools. BSA-seq revealed nine QTL: four for heading date, three for plant height, and two for panicle length. These QTL were validated in a random F2 population or BC4F2 populations. The major novel plant height QTL, qPH8, acting additively with an effect equivalent to that of semi-dwarf 1 (sd1), is potentially valuable for hybrid rice breeding. qPH8 controls mainly the elongation of basal internodes. The C7 allele of qPH8 reduces plant height and increases lodging resistance without yield penalty, suggesting a potential role for qPH8 in improving rice plant architecture.

Keywords

Lodging resistanceF2Bulked-segregant analysisBC4F2QTL validation

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The Crop Journal
Volume 9 Issue 6,
December 2021
Pages 1428-1437
DOI: 10.1016/j.cj.2020.11.011
Cite this article:
Zhang B, Qi F, Hu G, et al. BSA-seq-based identification of a major additive plant height QTL with an effect equivalent to that of Semi-dwarf 1 in a large rice F2 population. The Crop Journal, 2021, 9(6): 1428-1437. https://doi.org/10.1016/j.cj.2020.11.011

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Received: 24 August 2020
Revised: 17 November 2020
Accepted: 28 December 2020
Published: 26 January 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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