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Special Focus | Open Access

Mitochondrial localization of ORF346 causes pollen abortion in alloplasmic male sterility

Shifei Sanga,b,1, Hongtao Chenga,1, Mengyu Haoa, Bingli Dinga, Desheng Meia, Hui Wanga, Wenxiang Wanga, Jia Liua, Li Fua, Kede Liub, Qiong Hua( )
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China

1 These authors contributed equally to the work.

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Abstract

The Nsa cytoplasmic male sterility (CMS) system confers stable male sterility and offers great potential for production of hybrid seeds in oilseed rape. However, genes responsible for male sterility in Nsa CMS have not been identified. By mitochondrial genome sequencing of Nsa CMS and its maintainer line, we identified in an Nsa CMS line several chimeric genes encoding hypothetical proteins harboring transmembrane domains. One novel chimeric gene orf346 showed high identity with cox1 at the 5′ terminal region and was co-transcribed with nad3 and rps12 genes. Transgenic plants of orf346 fused with or without mitochondrial targeting peptide conferred complete male sterility in Arabidopsis. ORF346 was mitochondrion-localized. Expression of orf346 in Escherichia coli inhibited bacterial growth, with excessive accumulation of reactive oxygen species and decreased ATP content. These results reveal a link between the newly identified mitochondrial gene orf346 and the abortion of Nsa CMS. Inadequate energy supply and excessive accumulation of reactive oxygen species may account for pollen abortion in Nsa CMS plants.

Keywords

Reactive oxygen speciesATPBrassica napusCytoplasmic male sterilityOrf346

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The Crop Journal
Volume 9 Issue 6,
December 2021
Pages 1320-1329
DOI: 10.1016/j.cj.2021.01.008
Cite this article:
Sang S, Cheng H, Hao M, et al. Mitochondrial localization of ORF346 causes pollen abortion in alloplasmic male sterility. The Crop Journal, 2021, 9(6): 1320-1329. https://doi.org/10.1016/j.cj.2021.01.008

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Received: 08 August 2020
Revised: 20 November 2020
Accepted: 29 January 2021
Published: 06 April 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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