Abstract
Two potential BRASSINAZOLE RESISTANT 1 (BZR1) homologs were downregulated by brassinosteroids (BRs) in Setaria italica roots. Functional analysis showed that BR regulates the dephosphorylation and nuclear localization of SiBZR1 and that SiBZR1 binds conserved BZR1-recognizing cis elements. In comparison with the wild type, SiBZR1-overexpressing S. italica seedlings were more sensitive to BR-inhibited primary root growth and drought stress, indicating that SiBZR1 is a positive regulator of BR signaling and a negative regulator of drought tolerance in S. italica. PLETHORA-LIKE 1 (SiPLT-L1) was found to be a direct target gene of SiBZR1 in S. italica roots. The expression of SiPLT-L1 was downregulated by SiBZR1. SiPLT-L1-overexpressing S. italica was less sensitive to BR-inhibited root growth and more tolerant to drought stress, possibly owing to the upregulation of drought-inducible Dehydrin-family genes.