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Short Communication | Open Access

GmNMHC5 may promote nodulation via interaction with GmGAI in soybean

Wenting Wanga, Zhili Wanga, Wensheng Houb, Li Chenb, Bingjun Jiangb, Wenya Mab, Lijuan Baia, Wenwen Songb, Cailong Xub, Tianfu Hanb(), Yongjun Fenga(), Cunxiang Wub()
School of Life Science, Beijing Institute of Technology, Beijing 100081, China
MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Abstract

Soybean (Glycine max [L.] Merr.) is a food and oil crop whose growth and yield are influenced by root and nodule development. In the present study, GmNMHC5 was found to promote the formation of nodules in overexpressing mutants. In contrast, the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply. In 35S:GmNMHC5 mutants, expression levels of genes involved in nodulation were significantly up-regulated. Both in vitro and in vivo biochemical analyses showed that GmNMHC5 directly interacted with GmGAI (a DELLA protein), and the content of gibberellin 3 (GA3) in overexpressing mutants was lower than that in the wild type. These results revealed that GmNMHC5 participates in the classical GA signaling pathway, and may regulate the content of GA3 to match the optimal concentration required for nodule formation, thereby promoting nodulation by directly interacting with GmGAI. A model illustrating the mechanism by which GmNMHC5 promotes soybean nodulation is presented.

Keywords

Glycine maxGmNMHC5GmGAINodulationGA pathway

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The Crop Journal
Volume 10 Issue 1,
February 2022
Pages 273-279
DOI: 10.1016/j.cj.2021.03.019
Cite this article:
Wang W, Wang Z, Hou W, et al. GmNMHC5 may promote nodulation via interaction with GmGAI in soybean. The Crop Journal, 2022, 10(1): 273-279. https://doi.org/10.1016/j.cj.2021.03.019
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