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Research Article | Open Access

OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield

Jie Wanga,b, Renjing Wanc, Haipeng Nieb, Shaowu Xuec, Zhongming Fanga,b,c( )
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Agricultural Sciences, Guizhou University, Guiyang 550025, Guizhou, China
Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, Hubei, China
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
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Abstract

Rice has a large number of nitrate or peptide transporter family (NPF) genes, but the effects of most members on rice growth and development are unknown. We report that OsNPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of OsNPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars. OsNPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In cRNA-injected Xenopus laevis oocytes, OsNPF5.16 transport of nitrate at high nitrate concentration depended on pH. Overexpression of OsNPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of OsNPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of OsNPF5.16 expression showed the opposite effects. Regulating OsNPF5.16 expression has potential for improving rice grain yield.

Keywords

GrowthRiceNatural variationGrain yield OsNPF5.16 Nitrate transporter

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The Crop Journal
Volume 10 Issue 2,
April 2022
Pages 397-406
DOI: 10.1016/j.cj.2021.08.005
Cite this article:
Wang J, Wan R, Nie H, et al. OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield. The Crop Journal, 2022, 10(2): 397-406. https://doi.org/10.1016/j.cj.2021.08.005

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Received: 27 February 2021
Revised: 05 June 2021
Accepted: 20 August 2021
Published: 26 August 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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