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Research Article | Open Access

A lignified-layer bridge controlled by a single recessive gene is associated with high pod-shatter resistance in Brassica napus L.

Wen Chua,b,c, Jia Liua, Hongtao Chenga, Chao Lia, Li Fua, Wenxiang Wanga, Hui Wanga, Mengyu Haoa, Desheng Meia, Kede Liub, Qiong Hua,c( )
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory for Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Abstract

Pod shattering causes severe yield loss in rapeseed (Brassica napus L.) under modern agricultural practice. Identification of highly shatter-resistant germplasm is desirable for the development of rapeseed cultivars for mechanical harvesting. In the present study, an elite line OR88 with strong shatter resistance and a lignified-layer bridge (LLB) structure was identified. The LLB structure was unique to OR88 and co-segregated with high pod-shatter resistance. The LLB structure is differentiated at stage 12 of gynoecium development without any gynoecium defects. Genetic analysis showed that LLB is controlled by a single recessive gene. By BSA-Seq and map-based cloning, the resistance gene location was delimited to a 0.688 Mb region on chromosome C09. Transcriptome analysis suggested BnTCP8.C09 as the gene responsible for LLB. The expression of BnTCP.C09 was strongly downregulated in OR88, suppressing cell proliferation in the pod valve margin. KASP markers linked to the candidate gene were developed. This pod shatter-resistant line could be used in rapeseed breeding programs by direct transfer of the gene with the assistance of the DNA markers.

Keywords

KASP markerRapeseedBSA-SeqPod shatterLignified-layer bridge

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The Crop Journal
Volume 10 Issue 3,
June 2022
Pages 638-646
DOI: 10.1016/j.cj.2021.09.005
Cite this article:
Chu W, Liu J, Cheng H, et al. A lignified-layer bridge controlled by a single recessive gene is associated with high pod-shatter resistance in Brassica napus L.. The Crop Journal, 2022, 10(3): 638-646. https://doi.org/10.1016/j.cj.2021.09.005

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Received: 22 June 2021
Revised: 17 August 2021
Accepted: 08 September 2021
Published: 02 November 2021
© 2021 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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