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Research Article | Open Access

The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice

Qingwen Wanga, Lan Nia, Zhenzhen Cuia, Jingjing Jianga, Chao Chena, Mingyi Jianga,b( )
National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture/College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha 410128, Hunan, China
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Abstract

Rice (Oryza sativa L.) is a staple cereal for more than two thirds of the world's population. Soil salinity severely limits rice growth, development, and grain yield. It is desirable to elucidate the mechanism of rice's salt-stress response. As the major source of H2O2, NADPH oxidase (Rboh) is believed to be involved in salt-stress tolerance. However, the function and mechanism of rice Rboh in salt stress response remain unclear. In this study, we found that the expression of OsRbohA was up-regulated by NaCl treatment in the shoots and roots of rice seedlings. Knockout of OsRbohA reduced the tolerance of rice to salt stress. Knockout of OsRbohA blocked NaCl-induced increases of NADPH activity and H2O2 content in roots. OsRbohA knockout inhibited root growth and disrupted K+ homeostasis by reducing the expression of K+ transporters and channel-associated genes (OsGORK, OsAKT1, OsHAK1, and OsHAK5) in roots under NaCl treatment. Under NaCl treatment, OsRbohA knockout also reduced subcellular K+ contents of the plasma membrane and soluble fraction. Overexpression of OsRbohA increased the expression of K+ transporters and channel-associated genes and reduced the loss of K+ ions in roots. These results indicate that OsRbohA-mediated H2O2 accumulation modulates K+ homeostasis, thereby increasing salt tolerance in rice.

Keywords

RiceSalt toleranceH2O2 OsRbohAK+ homeostasis

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The Crop Journal
Volume 10 Issue 6,
December 2022
Pages 1611-1622
DOI: 10.1016/j.cj.2022.03.004
Cite this article:
Wang Q, Ni L, Cui Z, et al. The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice. The Crop Journal, 2022, 10(6): 1611-1622. https://doi.org/10.1016/j.cj.2022.03.004

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Received: 08 September 2021
Revised: 08 November 2021
Accepted: 05 April 2022
Published: 19 April 2022
© 2022 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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