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Research Article | Open Access

Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems

Dagang Tiana(), Yan Lina, Shengping Lib, Yiyang Caob, Gang Lia, Xinrui Guoa, Ziqiang Chena, Zaijie Chena, Feng Wanga(), Zonghua Wangb
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350003, Fujian, China
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Abstract

Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses. In rice, the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion (SL). Previous studies have identified an sl mutation (a null mutation of SL) in several rice varieties and confirmed its increase of resistance and cell death. However, a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking. We performed a multi-omics analysis to clarify the fundamental mechanisms at the protein, gene transcript, and metabolite levels. We found that cell death and Magnaporthe oryzae (M. oryzae) infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) in multiple regulatory layers. We characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance. SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M. oryzae. Our studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.

Keywords

Sekiguchi lesionCell deathPhytohormoneM. oryzaeRice

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The Crop Journal
Volume 10 Issue 6,
December 2022
Pages 1755-1766
DOI: 10.1016/j.cj.2022.03.005
Cite this article:
Tian D, Lin Y, Li S, et al. Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems. The Crop Journal, 2022, 10(6): 1755-1766. https://doi.org/10.1016/j.cj.2022.03.005
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