AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home The Crop Journal Article
PDF (2.4 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Short Communication | Open Access

Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding

Xuanjun Fenga,b,1, Yue Hub,1, Weixiao Zhangb, Rongqian Xieb, Huarui Guanb, Hao Xiongb, Li Jiab, Xuemei Zhangb, Hanmei Zhoub, Dan Zhengb, Ying Wenb, Qingjun Wangb, Fengkai Wub, Jie Xub, Yanli Lua,b( )
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang 611130, Sichuan, China
Maize Research Institute of Sichuan Agricultural University, Wenjiang 611130, Sichuan, China

1 These authors contributed equally to this work.

Show Author Information

Abstract

The delta-1-pyrroline-5-carboxylate synthetase (P5CS) gene exercises a protective function in stressed plants. However, the relationship between proline accumulation caused by P5CS and abiotic stress tolerance in plants is not always clear, as P5CS overexpression has been reported to repress plant growth under normal conditions in several reports. We re-evaluated the role of P5CS in drought-tolerant rice breeding by expressing the AtP5CS1 and feedback-inhibition-removed AtP5CS1 (AtP5CS1F128A) genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CS overexpression under normal conditions. ABA-inducible AtP5CS1 and AtP5CS1F128A increased seedling growth in a nutrient solution (under osmotic stress) and grain yield in pot plants. However, the evidently deleterious effects of AtP5CS1 on grain quality, tiller number, and grain yield in the field indicated the unsuitability of P5CS for drought-tolerance breeding.

Keywords

ProlineDroughtP5CSTiller numberChalkiness

References

[1]

L. Szabados, A. Savoure, Proline: a multifunctional amino acid, Trends Plant Sci. 15 (2010) 89-97.
Crossref Google Scholar
[2]

P.B.K. Kishor, Z. Hong, C.H. Miao, C.A.A. Hu, D.P.S. Verma, Overexpression of delta-1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants, Plant Physiol. 108 (1995) 1387-1394.
Crossref Google Scholar
[3]

P.B.K. Kishor, S. Sangam, R.N. Amrutha, P.S. Laxmi, K.R. Naidu, K.R.S.S. Rao, S. Rao, K.J. Reddy, P. Theriappan, N. Sreenivasulu, Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance, Curr. Sci. 88 (2005) 424-438.
Google Scholar
[4]

P. Surender Reddy, G. Jogeswar, G.K. Rasineni, M. Maheswari, A.R. Reddy, R.K. Varshney, P.B. Kavi Kishor, Proline over-accumulation alleviates salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum [Sorghum bicolor (L.) Moench], Plant Physiol. Biochem. 94 (2015) 104-113.
Crossref Google Scholar
[5]

B. Zhu, J. Su, M. Chang, D.P.S. Verma, Y.L. Fan, R. Wu, Overexpression of a Δ1-pyrroline-5-carboxylate synthetase gene and analysis of tolerance to water- and salt-stress in transgenic rice, Plant Sci. 139 (1998) 41-48.
Crossref Google Scholar
[6]

P. Bhatnagar-Mathur, V. Vadez, M. Jyostna Devi, M. Lavanya, G. Vani, K.K. Sharma, Genetic engineering of chickpea (Cicer arietinum L.) with the P5CSF129A gene for osmoregulation with implications on drought tolerance, Mol. Breed. 23 (2009) 591-606.
Crossref Google Scholar
[7]

C.h. Surekha, K.N. Kumari, L.V. Aruna, G. Suneetha, A. Arundhati, P.B. Kavi Kishor, Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance, Plant Cell Tiss. Org. 116 (2014) 27-36.
Crossref Google Scholar
[8]

V. Kumar, V. Shriram, P.B. Kavi Kishor, N. Jawali, M.G. Shitole, Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene, Plant Biotechnol. Rep. 4 (2010) 37-48.
Crossref Google Scholar
[9]

J. Su, R. Wu, RayWu, Stress-inducible synthesis of proline in transgenic rice confers faster growth under stress conditions than that with constitutive synthesis, Plant Sci. 166 (2004) 941-948.
Crossref Google Scholar
[10]

N. Anoop, A.K. Gupta, Transgenic indica rice cv IR-50 over-expressing vigna aconitifolia Δ1-pyrroline -5- carboxylate synthetase cDNA shows tolerance to high salt, J. Plant Biochem. Biotechnol. 12 (2003) 109-116.
Crossref Google Scholar
[11]

A. Hmida-Sayari, R. Gargouri-Bouzid, A. Bidani, L. Jaoua, A. Savouré, S. Jaoua, Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants, Plant Sci. 169 (2005) 746-752.
Crossref Google Scholar
[12]

C.A. Hu, A.J. Delauney, D.P. Verma, A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants, Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 9354-9358.
Crossref Google Scholar
[13]

C.S. Zhang, Q. Lu, D.P.S. Verma, Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate Synthetase, a Bifunctional enzyme catalyzing the first two steps of proline biosynthesis in plants, J. Biol. Chem. 270 (1995) 20491-20496.
Crossref Google Scholar
[14]

M. Xi, W. Wu, Y. Xu, Y. Zhou, G. Chen, Y. Ji, X. Sun, Grain chalkiness traits is affected by the synthesis and dynamic accumulation of the storage protein in rice, J. Sci. Food. Agric. 101 (2021) 6125-6133.
Crossref Google Scholar
[15]

Z. Lin, Z. Wang, X. Zhang, Z. Liu, G. Li, S. Wang, Y. Ding, Complementary proteome and transcriptome profiling in developing grains of a notched-belly rice mutant reveals key pathways involved in chalkiness formation, Plant Cell Physiol. 58 (2017) 560–573.
Crossref Google Scholar
[16]

Y.S. Chen, S.F. Lo, P.K. Sun, C.A. Lu, T.H. Ho, S.M. Yu, A late embryogenesis abundant protein HVA1 regulated by an inducible promoter enhances root growth and abiotic stress tolerance in rice without yield penalty, Plant Biotechnol. J. 13 (2015) 105-116.
Crossref Google Scholar
[17]

M.J. Kovach, M.N. Calingacion, M.A. Fitzgerald, S.R. McCouch, The origin and evolution of fragrance in rice (Oryza sativa L.), Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 14444-14449.
Crossref Google Scholar
[18]

M.A.J.S. van Boekel, Formation of flavour compounds in the Maillard reaction, Biotechnol. Adv. 24 (2006) 230-233.
Crossref Google Scholar
[19]

I. Blank, S. Devaud, W. Matthey-Doret, F. Robert, Formation of odorants in maillard model systems based on l-proline as affected by pH, J. Agric. Food Chem. 51 (2003) 3643-3650.
Crossref Google Scholar
[20]

K. Kaewka, C. Therakulkait, K.R. Cadwallader, Effect of drying and proline addition on aroma volatile compounds of acid-hydrolysed rice bran protein concentrate, Int. J. Food Sci. Technol. 46 (2011) 1654–1660.
Crossref Google Scholar
[21]

X.J. Feng, J.R. Li, S.L. Qi, Q.F. Lin, J.B. Jin, X.J. Hua, Light affects salt stress-induced transcriptional memory of P5CS1 in Arabidopsis, Proc. Natl. Acad. Sci. U. S. A. 113 (2016) E8335-E8343.
Crossref Google Scholar
[22]

M. Tabuchi, K. Sugiyama, K. Ishiyama, E. Inoue, T. Sato, H. Takahashi, T. Yamaya, Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1, Plant J. 42 (2005) 641–651.
Crossref Google Scholar
[23]

M. Tabuchi, T. Abiko, T. Yamaya, Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.), J. Exp. Bot. 58 (2007) 2319-2327.
Crossref Google Scholar
[24]

K. Funayama, S. Kojima, M. Tabuchi-Kobayashi, Y. Sawa, Y. Nakayama, T. Hayakawa, T. Yamaya, Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots, Plant Cell Physiol. 54 (2013) 934–943.
Crossref Google Scholar
[25]

B.Z. Xiao, X.I. Chen, C.B. Xiang, N. Tang, Q.F. Zhang, L.Z. Xiong, Evaluation of seven function-known candidate genes for their effects on improving drought resistance of transgenic rice under field conditions, Mol. Plant 2 (2009) 73-83.
Crossref Google Scholar
[26]

J. Deikman, M. Petracek, J.E. Heard, Drought tolerance through biotechnology: improving translation from the laboratory to farmers’ fields, Curr. Opin. Biotechnol. 23 (2012) 243-250.
Crossref Google Scholar
The Crop Journal
Volume 10 Issue 4,
August 2022
Pages 1213-1218
DOI: 10.1016/j.cj.2022.04.002
Cite this article:
Feng X, Hu Y, Zhang W, et al. Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding. The Crop Journal, 2022, 10(4): 1213-1218. https://doi.org/10.1016/j.cj.2022.04.002

256

Views

3

Downloads

6

Crossref

5

Web of Science

7

Scopus

2

CSCD

Altmetrics
Received: 30 January 2022
Revised: 06 April 2022
Accepted: 10 April 2022
Published: 02 May 2022
© 2022 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return