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Research Article | Open Access

HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley

Deshui Yua,b,c( ), Renchun Fanb, Ling Zhangb, Pengya Xueb,c, Libing Liaoa, Meizhen Hub, Yanjun Chengb, Jine Lib,c, Ting Qib, Shaojuan Jingb, Qiuyun Wangb, Arvind Bhatta, Qian-Hua Shenb,c( )
Lushan Botanical Garden, Chinese Academy of Science, Jiujiang 332900, Jiangxi, China
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Innovation Academy for Seed Design, Beijing 100101, China
CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract

Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner. In barley, HvWRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus, Blumeria graminis f. sp. hordei (Bgh). However, the molecular features of HvWRKY2 in its DNA-binding and repressor functions, as well as its target genes, are uncharacterized. We show that the W-box binding of HvWRKY2 requires an intact WRKY domain and an upstream sequence of ~75 amino acids, and the HvWRKY2 W-box binding activity is linked to its repressor function in disease resistance. Chromatin immunoprecipitation (ChIP)-seq analysis identified HvCEBiP, a putative chitin receptor gene, as a target gene of HvWRKY2 in overexpressing transgenic barley plants. ChIP-qPCR and Electrophoretic Mobility Shift Assay (EMSA) verified the direct binding of HvWRKY2 to a W-box-containing sequence in the HvCEBiP promoter. HvCEBiP positively regulates resistance against Bgh in barley. Our findings suggest that HvWRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern (PAMP) recognition receptor genes, suggesting that HvCEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection.

Keywords

HvWRKY2Basal immunityChromatin immunoprecipitation (ChIP)HvCEBiP

References

[1]

W. Wang, B. Feng, J.M. Zhou, D. Tang, Plant immune signaling: advancing on two frontiers, J. Integr. Plant Biol. 62 (2020) 2–24.
Crossref Google Scholar
[2]

A.R. Bentham, J.C. de la Concepcion, N. Mukhi, R. Zdrzalek, M. Draeger, D. Gorenkin, R.K. Hughes, M.J. Banfield, A molecular roadmap to the plant immune system, J. Biol. Chem. 295 (2020) 14916–14935.
Crossref Google Scholar
[3]

J.D. Jones, J.L. Dangl, The plant immune system, Nature 444 (2006) 323–329.
Crossref Google Scholar
[4]

D. Tang, G. Wang, J.M. Zhou, Receptor kinases in plant-pathogen interactions: more than pattern recognition, Plant Cell 29 (2017) 618–637.
Crossref Google Scholar
[5]

C. Zipfel, Plant pattern-recognition receptors, Trends Immunol. 35 (2014) 345–351.
Crossref Google Scholar
[6]

Y. Wu, J. Zhou, Receptor-like kinases in plant innate immunity, J. Integr. Plant Biol. 55 (2013) 1271–1286.
Crossref Google Scholar
[7]

C. Zipfel, Early molecular events in PAMP-triggered immunity, Curr. Opin. Plant Biol. 12 (2009) 414–420.
Crossref Google Scholar
[8]

X.F. Xin, B. Kvitko, S.Y. He, Pseudomonas syringae: what it takes to be a pathogen, Nat. Rev. Microbiol. 16 (2018) 316–328.
Crossref Google Scholar
[9]

H. Cui, K. Tsuda, J.E. Parker, Effector-triggered immunity: from pathogen perception to robust defense, Annu. Rev. Plant Biol. 66 (2015) 487–511.
Crossref Google Scholar
[10]

S.T. Chisholm, G. Coaker, B. Day, B.J. Staskawicz, Host-microbe interactions: shaping the evolution of the plant immune response, Cell 124 (2006) 803–814.
Crossref Google Scholar
[11]

M. Yuan, B.P.M. Ngou, P. Ding, X.F. Xin, PTI-ETI crosstalk: an integrative view of plant immunity, Curr. Opin. Plant Biol. 62 (2021) 102030.
Crossref Google Scholar
[12]

D.E. Cook, C.H. Mesarich, B.P. Thomma, Understanding plant immunity as a surveillance system to detect invasion, Annu. Rev. Phytopathol. 53 (2015) 541–563.
Crossref Google Scholar
[13]

K. Tsuda, F. Katagiri, Comparing signaling mechanisms engaged in pattern-triggered and effector-triggered immunity, Curr. Opin. Plant Biol. 13 (2010) 459–465.
Crossref Google Scholar
[14]

M. Chang, H. Chen, F. Liu, Z.Q. Fu, PTI and ETI: convergent pathways with diverse elicitors, Trends Plant Sci. 27 (2022) 113–115.
Crossref Google Scholar
[15]

B.P.M. Ngou, H.K. Ahn, P. Ding, J.D.G. Jones, Mutual potentiation of plant immunity by cell-surface and intracellular receptors, Nature 592 (2021) 110–115.
Crossref Google Scholar
[16]

R.N. Pruitt, F. Locci, F. Wanke, L. Zhang, S.C. Saile, A. Joe, D. Karelina, C. Hua, K. Frohlich, W.L. Wan, M. Hu, S. Rao, S.C. Stolze, A. Harzen, A.A. Gust, K. Harter, M. Joosten, B. Thomma, J.M. Zhou, J.L. Dangl, D. Weigel, H. Nakagami, C. Oecking, F.E. Kasmi, J.E. Parker, T. Nurnberger, The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity, Nature 598 (2021) 495–499.
Crossref Google Scholar
[17]

H. Tian, Z. Wu, S. Chen, K. Ao, W. Huang, H. Yaghmaiean, T. Sun, F. Xu, Y. Zhang, S. Wang, X. Li, Y. Zhang, Activation of TIR signalling boosts pattern-triggered immunity, Nature 598 (2021) 500–503.
Crossref Google Scholar
[18]

Z. Wu, L. Tian, X. Liu, Y. Zhang, X. Li, TIR signal promotes interactions between lipase-like proteins and ADR1-L1 receptor and ADR1-L1 oligomerization, Plant Physiol. 187 (2021) 681–686.
Crossref Google Scholar
[19]

N. Shibuya, E. Minami, Oligosaccharide signalling for defence responses in plant, Physiol. Mol. Plant P. 59 (2001) 223–233.
Crossref Google Scholar
[20]

B.Q. Gong, F.Z. Wang, J.F. Li, Hide-and-seek: chitin-triggered plant immunity and fungal counterstrategies, Trends Plant Sci. 25 (2020) 805–816.
Crossref Google Scholar
[21]

H. Kaku, Y. Nishizawa, N. Ishii-Minami, C. Akimoto-Tomiyama, N. Dohmae, K. Takio, E. Minami, N. Shibuya, Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor, Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 11086–11091.
Crossref Google Scholar
[22]

T. Shimizu, T. Nakano, D. Takamizawa, Y. Desaki, N. Ishii-Minami, Y. Nishizawa, E. Minami, K. Okada, H. Yamane, H. Kaku, N. Shibuya, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, Plant J. 64 (2010) 204–214.
Crossref Google Scholar
[23]

Y. Ao, Z. Li, D. Feng, F. Xiong, J. Liu, J.F. Li, M. Wang, J. Wang, B. Liu, H.B. Wang, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, Plant J. 80 (2014) 1072–1084.
Crossref Google Scholar
[24]

Y. Kouzai, S. Mochizuki, K. Nakajima, Y. Desaki, M. Hayafune, H. Miyazaki, N. Yokotani, K. Ozawa, E. Minami, H. Kaku, N. Shibuya, Y. Nishizawa, Targeted gene disruption of OsCERK1 reveals its indispensable role in chitin perception and involvement in the peptidoglycan response and immunity in rice, Mol. Plant-Microbe Interact. 27 (2014) 975–982.
Crossref Google Scholar
[25]

Y. Kouzai, K. Nakajima, M. Hayafune, K. Ozawa, H. Kaku, N. Shibuya, E. Minami, Y. Nishizawa, CEBiP is the major chitin oligomer-binding protein in rice and plays a main role in the perception of chitin oligomers, Plant Mol. Biol. 84 (2014) 519–528.
Crossref Google Scholar
[26]

S. Tanaka, A. Ichikawa, K. Yamada, G. Tsuji, T. Nishiuchi, M. Mori, H. Koga, Y. Nishizawa, R. O'Connell, Y. Kubo, HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnaporthe oryzae, BMC Plant Biol. 10 (2010) 288.
Crossref Google Scholar
[27]

S.P. Pandey, I.E. Somssich, The role of WRKY transcription factors in plant immunity, Plant Physiol. 150 (2009) 1648–1655.
Crossref Google Scholar
[28]

T. Eulgem, I.E. Somssich, Networks of WRKY transcription factors in defense signaling, Curr. Opin. Plant Biol. 10 (2007) 366–371.
Crossref Google Scholar
[29]

T. Eulgem, Dissecting the WRKY web of plant defense regulators, PLoS Pathog. 2 (2006) e126.
Crossref Google Scholar
[30]

B. Ulker, I.E. Somssich, WRKY transcription factors: from DNA binding towards biological function, Curr. Opin. Plant Biol. 7 (2004) 491–498.
Crossref Google Scholar
[31]

T. Eulgem, P.J. Rushton, S. Robatzek, I.E. Somssich, The WRKY superfamily of plant transcription factors, Trends Plant Sci. 5 (2000) 199–206.
Crossref Google Scholar
[32]

Y.P. Xu, H. Xu, B. Wang, X.D. Su, Crystal structures of N-terminal WRKY transcription factors and DNA complexes, Protein Cell 11 (2020) 208–213.
Crossref Google Scholar
[33]

P.J. Rushton, I.E. Somssich, P. Ringler, Q.J. Shen, WRKY transcription factors, Trends Plant Sci. 15 (2010) 247–258.
Crossref Google Scholar
[34]

K.L. Wu, Z.J. Guo, H.H. Wang, J. Li, The WRKY family of transcription factors in rice and Arabidopsis and their origins, DNA Res. 12 (2005) 9–26.
Crossref Google Scholar
[35]

T. Eulgem, P.J. Rushton, E. Schmelzer, K. Hahlbrock, I.E. Somssich, Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors, EMBO J. 18 (1999) 4689–4699.
Crossref Google Scholar
[36]

P.J. Rushton, J.T. Torres, M. Parniske, P. Wernert, K. Hahlbrock, I.E. Somssich, Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes, EMBO J. 15 (1996) 5690–5700.
Crossref Google Scholar
[37]

K. Tsuda, I.E. Somssich, Transcriptional networks in plant immunity, New Phytol. 206 (2015) 932–947.
Crossref Google Scholar
[38]

R.P. Birkenbihl, B. Kracher, M. Roccaro, I.E. Somssich, Induced genome-wide binding of three Arabidopsis WRKY transcription factors during early MAMP-triggered immunity, Plant Cell 29 (2017) 20–38.
Crossref Google Scholar
[39]

M. Schon, A. Toller, C. Diezel, C. Roth, L. Westphal, M. Wiermer, I.E. Somssich, Analyses of wrky18 wrky40 plants reveal critical roles of SA/EDS1 signaling and indole-glucosinolate biosynthesis for Golovinomyces orontii resistance and a loss-of resistance towards Pseudomonas syringae pv. tomato AvrRPS4, Mol. Plant-Microbe Interact. 26 (2013) 758–767.
Crossref Google Scholar
[40]

S.P. Pandey, M. Roccaro, M. Schon, E. Logemann, I.E. Somssich, Transcriptional reprogramming regulated by WRKY18 and WRKY40 facilitates powdery mildew infection of Arabidopsis, Plant J. 64 (2010) 912–923.
Crossref Google Scholar
[41]

Q.H. Shen, Y. Saijo, S. Mauch, C. Biskup, S. Bieri, B. Keller, H. Seki, B. Ulker, I.E. Somssich, P. Schulze-Lefert, Nuclear activity of MLA immune receptors links isolate-specific and basal disease-resistance responses, Science 315 (2007) 1098–1103.
Crossref Google Scholar
[42]

X. Xu, C. Chen, B. Fan, Z. Chen, Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors, Plant Cell 18 (2006) 1310–1326.
Crossref Google Scholar
[43]

Y. Peng, L.E. Bartley, X. Chen, C. Dardick, M. Chern, R. Ruan, P.E. Canlas, P.C. Ronald, OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice, Mol. Plant 1 (2008) 446–458.
Crossref Google Scholar
[44]

J. Liu, X. Chen, X. Liang, X. Zhou, F. Yang, J. Liu, S.Y. He, Z. Guo, Alternative splicing of rice WRKY62 and WRKY76 transcription factor genes in pathogen defense, Plant Physiol. 171 (2016) 1427–1442.
Crossref Google Scholar
[45]

T. Chujo, K. Miyamoto, T. Shimogawa, T. Shimizu, Y. Otake, N. Yokotani, Y. Nishizawa, N. Shibuya, H. Nojiri, H. Yamane, E. Minami, K. Okada, OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus, Plant Mol. Biol. 82 (2013) 23–37.
Crossref Google Scholar
[46]

X. Han, L. Zhang, L. Zhao, P. Xue, T. Qi, C. Zhang, H. Yuan, L. Zhou, D. Wang, J. Qiu, Q.H. Shen, SnRK1 phosphorylates and destabilizes WRKY3 to enhance barley immunity to powdery mildew, Plant Commun. 1 (2020) 100083.
Crossref Google Scholar
[47]

S. Bai, J. Liu, C. Chang, L. Zhang, T. Maekawa, Q. Wang, W. Xiao, Y. Liu, J. Chai, F.L. Takken, P. Schulze-Lefert, Q.H. Shen, Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance, PLoS Pathog. 8 (2012) e1002752.
Crossref Google Scholar
[48]

S. Seeholzer, T. Tsuchimatsu, T. Jordan, S. Bieri, S. Pajonk, W. Yang, A. Jahoor, K.K. Shimizu, B. Keller, P. Schulze-Lefert, Diversity at the Mla powdery mildew resistance locus from cultivated barley reveals sites of positive selection, Mol. Plant-Microbe Interact. 23 (2010) 497–509.
Crossref Google Scholar
[49]

C. Chang, D. Yu, J. Jiao, S. Jing, P. Schulze-Lefert, Q.H. Shen, Barley MLA immune receptors directly interfere with antagonistically acting transcription factors to initiate disease resistance signaling, Plant Cell 25 (2013) 1158–1173.
Crossref Google Scholar
[50]

J.G. Bartlett, S.C. Alves, M. Smedley, J.W. Snape, W.A. Harwood, High-throughput Agrobacterium-mediated barley transformation, Plant Methods 4 (2008) 22.
Crossref Google Scholar
[51]

D. Yu, L. Liao, Y. Zhang, K. Xu, J. Zhang, K. Liu, X. Li, G. Tan, J. Zheng, Y. He, C. Xu, J. Zhao, B. Fu, J. Xie, J. Mao, C. Li, Development of a Gateway-compatible pCAMBIA binary vector for RNAi-mediated gene knockdown in plants, Plasmid 98 (2018) 52–55.
Crossref Google Scholar
[52]

J. Zhang, D. Yu, Y. Zhang, K. Liu, K. Xu, F. Zhang, J. Wang, G. Tan, X. Nie, Q. Ji, L. Zhao, C. Li, Vacuum and co-cultivation agroinfiltration of (germinated) seeds results in Tobacco rattle virus (trv) mediated whole-plant virus-induced gene silencing (vigs) in wheat and maize, Front. Plant Sci. 8 (2017) 393.
Crossref Google Scholar
[53]

T. Wang, C. Chang, C. Gu, S. Tang, Q. Xie, Q.H. Shen, An E3 ligase affects the NLR receptor stability and immunity to powdery mildew, Plant Physiol. 172 (2016) 2504–2515.
Crossref Google Scholar
[54]

J. Liu, X. Cheng, D. Liu, W. Xu, R. Wise, Q.H. Shen, The miR9863 family regulates distinct Mla alleles in barley to attenuate NLR receptor-triggered disease resistance and cell-death signaling, PLoS Genet. 10 (2014) e1004755.
Crossref Google Scholar
[55]

D. Yu, L. Liao, J. Zhang, Y. Zhang, K. Xu, K. Liu, X. Li, G. Tan, R. Chen, Y. Wang, X. Liu, X. Zhang, X. Han, Z. Wei, C. Li, A novel, easy and rapid method for constructing yeast two-hybrid vectors using In-Fusion technology, BioTechniques 64 (2018) 219–224.
Crossref Google Scholar
[56]

M. Roccaro, I.E. Somssich, Chromatin immunoprecipitation to identify global targets of WRKY transcription factor family members involved in plant immunity, Methods Mol. Biol. 712 (2011) 45–58.
Crossref Google Scholar
[57]

S. Guo, Y. Xu, H. Liu, Z. Mao, C. Zhang, Y. Ma, Q. Zhang, Z. Meng, K. Chong, The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14, Nat. Commun. 4 (2013) 1566.
Crossref Google Scholar
[58]

W. Wei, J. Huang, Y.J. Hao, H.F. Zou, H.W. Wang, J.Y. Zhao, X.Y. Liu, W.K. Zhang, B. Ma, J.S. Zhang, S.Y. Chen, Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants, PLoS ONE 4 (2009) e7209.
Crossref Google Scholar
[59]

S. de Pater, V. Greco, K. Pham, J. Memelink, J. Kijne, Characterization of a zinc-dependent transcriptional activator from Arabidopsis, Nucleic Acids Res. 24 (1996) 4624–4631.
Crossref Google Scholar
[60]

Q.H. Shen, F. Zhou, S. Bieri, T. Haizel, K. Shirasu, P. Schulze-Lefert, Recognition specificity and RAR1/SGT1 dependence in barley Mla disease resistance genes to the powdery mildew fungus, Plant Cell 15 (2003) 732–744.
Crossref Google Scholar
[61]

D. Douchkov, D. Nowara, U. Zierold, P. Schweizer, A high-throughput gene-silencing system for the functional assessment of defense-related genes in barley epidermal cells, Mol. Plant-Microbe Interact. 18 (2005) 755–761.
Crossref Google Scholar
[62]

F. Delplace, C. Huard-Chauveau, R. Berthome, D. Roby, Network organization of the plant immune system: from pathogen perception to robust defense induction, Plant J. 109 (2022) 447–470.
Crossref Google Scholar
[63]

X. Yu, B. Li, G.J. Jang, S. Jiang, D. Jiang, J.C. Jang, S.H. Wu, L. Shan, P. He, Orchestration of processing body dynamics and mRNA decay in Arabidopsis immunity, Cell Rep. 28 (2019) 2194–2205.
Crossref Google Scholar
[64]

C. Chang, L. Zhang, Q.H. Shen, Partitioning, repressing and derepressing: dynamic regulations in MLA immune receptor triggered defense signaling, Front. Plant Sci. 4 (2013) 396.
Crossref Google Scholar
[65]

K. Maeo, S. Hayashi, H. Kojima-Suzuki, A. Morikami, K. Nakamura, Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins, Biosci. Biotechnol. Biochem. 65 (2001) 2428–2436.
Crossref Google Scholar
[66]

K. Yamasaki, T. Kigawa, S. Watanabe, M. Inoue, T. Yamasaki, M. Seki, K. Shinozaki, S. Yokoyama, Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor, J. Biol. Chem. 287 (2012) 7683–7691.
Crossref Google Scholar
[67]

M.R. Duan, J. Nan, Y.H. Liang, P. Mao, L. Lu, L. Li, C. Wei, L. Lai, Y. Li, X.D. Su, DNA binding mechanism revealed by high resolution crystal structure of Arabidopsis thaliana WRKY1 protein, Nucleic Acids Res. 35 (2007) 1145–1154.
Crossref Google Scholar
[68]

K. Yamasaki, T. Kigawa, M. Inoue, M. Tateno, T. Yamasaki, T. Yabuki, M. Aoki, E. Seki, T. Matsuda, Y. Tomo, N. Hayami, T. Terada, M. Shirouzu, A. Tanaka, M. Seki, K. Shinozaki, S. Yokoyama, Solution structure of an Arabidopsis WRKY DNA binding domain, Plant Cell 17 (2005) 944–956.
Crossref Google Scholar
[69]

S. Ishiguro, K. Nakamura, Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5' upstream regions of genes coding for sporamin and beta-amylase from sweet potato, Mol. Gen. Genet. 244 (1994) 563–571.
Crossref Google Scholar
[70]

Y. Kawano, K. Shimamoto, Early signaling network in rice PRR-mediated and R-mediated immunity, Curr. Opin. Plant Biol. 16 (2013) 496–504.
Crossref Google Scholar
[71]

A. Akamatsu, H.L. Wong, M. Fujiwara, J. Okuda, K. Nishide, K. Uno, K. Imai, K. Umemura, T. Kawasaki, Y. Kawano, K. Shimamoto, An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity, Cell Host Microbe 13 (2013) 465–476.
Crossref Google Scholar
[72]

K. Yamaguchi, K. Yamada, K. Ishikawa, S. Yoshimura, N. Hayashi, K. Uchihashi, N. Ishihama, M. Kishi-Kaboshi, A. Takahashi, S. Tsuge, A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity, Cell Host Microbe 13 (2013) 347–357.
Crossref Google Scholar
[73]

K. Kishimoto, Y. Kouzai, H. Kaku, N. Shibuya, E. Minami, Y. Nishizawa, Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice, Plant J. 64 (2010) 343–354.
Crossref Google Scholar
[74]

C.O. Micali, U. Neumann, D. Grunewald, R. Panstruga, R. O'Connell, Biogenesis of a specialized plant-fungal interface during host cell internalization of Golovinomyces orontii haustoria, Cell Microbiol. 13 (2011) 210–226.
Crossref Google Scholar
[75]

S. Karre, A. Kumar, D. Dhokane, A.C. Kushalappa, Metabolo-transcriptome profiling of barley reveals induction of chitin elicitor receptor kinase gene (HvCERK1) conferring resistance against Fusarium graminearum, Plant Mol. Biol. 93 (2017) 247–267.
Crossref Google Scholar
[76]

A. Polonio, D. Fernandez-Ortuno, A. de Vicente, A. Perez-Garcia, A haustorial-expressed lytic polysaccharide monooxygenase from the cucurbit powdery mildew pathogen Podosphaera xanthii contributes to the suppression of chitin-triggered immunity, Mol. Plant Pathol. 22 (2021) 580–601.
Crossref Google Scholar
The Crop Journal
Volume 11 Issue 1,
February 2023
Pages 99-107
DOI: 10.1016/j.cj.2022.05.010
Cite this article:
Yu D, Fan R, Zhang L, et al. HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley. The Crop Journal, 2023, 11(1): 99-107. https://doi.org/10.1016/j.cj.2022.05.010

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Received: 29 January 2022
Revised: 17 April 2022
Accepted: 17 June 2022
Published: 03 July 2022
© 2022 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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