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Short Communication | Open Access

A betaxanthin-based visible and fluorescent reporter for monitoring plant transformation

Yuanjie Deng1Aoqi Duan1Tong LiHuiru WangAisheng Xiong( )
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

1 These authors contributed equally to this work.

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Abstract

Commonly used reporters rely on a single property, such as the fluorescence of GFP and visible color of anthocyanins, therefore these reporters hardly handle the complicated condition in practice. Betaxanthins are a group of plant natural products derived from the amino acid tyrosine. Its visible yellow-orange color and green fluorescence under blue light make it a promising new reporter. Only two enzymatic reactions are required to convert tyrosine into betaxanthins. Here, we synthesized an open reading frame named Bx that contained all the betaxanthins biosynthetic genes and demonstrated its use as a powerful and efficient reporter in tobacco, carrot, and tomato.

The Crop Journal
Pages 666-671
Cite this article:
Deng Y, Duan A, Li T, et al. A betaxanthin-based visible and fluorescent reporter for monitoring plant transformation. The Crop Journal, 2023, 11(2): 666-671. https://doi.org/10.1016/j.cj.2022.10.010

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Received: 26 August 2022
Revised: 26 September 2022
Accepted: 29 November 2022
Published: 05 December 2022
© 2022 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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