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Research paper | Open Access

Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice

Siliang Xua,1, Mengchen Zhanga,1, Junhua Yea, Dongxiu Hua, Yuanyuan Zhanga, Zhen Lia, Junrong Liua, Yanfei Suna, Shan Wanga, Xiaoping Yuana, Yue Fenga, Qun Xua, Xinghua Weia( ), Dali Zengb( ), Yaolong Yanga( )
China National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A & F University, Hangzhou 311300, Zhejiang, China

1 These authors contributed equally to this work.

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Abstract

Because plant mechanical strength influences plant growth and development, the regulatory mechanisms underlying cell-wall synthesis deserve investigation. Rice mutants are useful for such research. We have identified a novel brittle culm 25 (bc25) mutant with reduced growth and partial sterility. BC25 encodes an UDP-glucuronic acid decarboxylase involved in cellulose synthesis and belongs to the UXS family. A single-nucleotide mutation in BC25 accounts for its altered cell morphology and cell-wall composition. Transmission electron microscopy analysis showed that the thickness of the secondary cell wall was reduced in bc25. Monosaccharide analysis revealed significant increases in content of rhamnose and arabinose but not of other monosaccharides, indicating that BC25 was involved in xylose synthesis with some level of functional redundancy. Enzymatic assays suggested that BC25 functions with high activity to interconvert UDP-glucuronic acid (UDP-GlcA) and UDP-xylose. GUS staining showed that BC25 was ubiquitously expressed with higher expression in culm, root and sheath, in agreement with that shown by quantitative real-time (qRT)-PCR. RNA-seq further suggested that BC25 is involved in sugar metabolism. We conclude that BC25 strongly influences rice cell wall formation.

Keywords

Mechanical strengthRiceCell wallBrittle culmUDP-xylose

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The Crop Journal
Volume 11 Issue 3,
June 2023
Pages 733-743
DOI: 10.1016/j.cj.2022.11.011
Cite this article:
Xu S, Zhang M, Ye J, et al. Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice. The Crop Journal, 2023, 11(3): 733-743. https://doi.org/10.1016/j.cj.2022.11.011

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Received: 09 June 2022
Revised: 31 October 2022
Accepted: 25 November 2022
Published: 02 January 2023
© 2022 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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