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Research paper | Open Access

The KL system in wheat permits homoeologous crossing over between closely related chromosomes

Chaolan Fana,b,c, Jiangtao Luod, Jiaojiao Sunc, Hong Chenc, Liqiong Lic, Lanyue Zhangc, Xue Chenc, Yazhou Lia, Shunzong Ningc, Zhongwei Yuanc, Bo Jiangc, Lianquan Zhanga, Xuejiao Chenc, Adam J. Lukaszewskib(), Dengcai Liua,c(), Ming Haoa()
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Crop Research Institute, Sichuan Academic of Agricultural Science, Chengdu 610066, Sichuan, China
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Abstract

The Chinese wheat landrace Kaixianluohanmai (KL) expresses the ph-like phenotype. A major QTL, QPh.sicau-3A (syn. phKL), responsible for this effect has been mapped to chromosome arm 3AL. This study presents some characteristics of homoeologous pairing and recombination induced by phKL. In KL haploids, the level of homoeologous pairing was elevated relative to Ph1 Chinese Spring (CS) haploids. There was a clear preference for A–D pairing and less frequent for A–B and B–D, reflecting the higher levels of affinity between genomes A and D in wheat. The characteristics of pairing were affected by temperature and magnesium ion supplementation. The suitability of phKL for chromosome engineering was tested on three pairs of homoeologues: 2Sv-2B, 2Sv-2D, and 2RL-2BL. The recombination rates were 1.68%, 0.17%, and 0%, respectively. The phKL locus in KL induced a moderate level of homoeologous chromosome pairing and recombination when the Ph1 locus of wheat was present, both in wheat haploids and hexaploids. The Ph1-imposed criteria for chromosome pairing and crossing over were relaxed to some degree, permitting homoeologous crossing over but only between closely related chromosomes; there was no crossing over between more differentiated chromosomes. Therefore, the phKL system (QPh.sicau-3A) can be a useful tool in chromosome engineering of wheat to transfer genes from closely related species with the benefit of reduced genomic chaos generated by the ph1b mutation.

Keywords

ph-like genephKLHomoeologous recombinationHomoeology

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The Crop Journal
Volume 11 Issue 3,
June 2023
Pages 808-816
DOI: 10.1016/j.cj.2023.01.003
Cite this article:
Fan C, Luo J, Sun J, et al. The KL system in wheat permits homoeologous crossing over between closely related chromosomes. The Crop Journal, 2023, 11(3): 808-816. https://doi.org/10.1016/j.cj.2023.01.003
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