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Research paper | Open Access

Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.)

Deyuan Menga,g,1, Aamana Batoola,g,1, Yazhou Xuanb, Ruiqing Pana, Na Zhanga, Wei Zhanga, Liya Zhia,g, Xiaoli Rena, Wenqing Lib, Jijie Lib, Yanxiao Niub, Shuzhi Zhengb,f, Jun Jia,d, Xiaoli Shid, Lei Wanga,d,f, Hongqing Lingd,g, Chunhua Zhaoe, Fa Cuie( ), Xigang Liub,f, Junming Lia,b,f( ), Liqiang Songc,f( )
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Shijiazhuang 050022, Hebei, China
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China
State Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding 071000, Hebei, China
State Key Laboratory of Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing 100101, China
Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, School of Agriculture, Ludong University, Yantai 264025, Shandong, China
Hebei Collaboration Innovation Center for Cell Signaling, Shijiazhuang 050024, Hebei, China
College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China

1 These authors contributed equally to this work.

Show Author Information

Abstract

Thousand-kernel weight (TKW) is a measure of grain weight, a target of wheat breeding. The object of this study was to fine-map a stable quantitative trait loci (QTL) for TKW and identify its candidate gene in a recombinant inbred line (RIL) population derived from the cross of Kenong 9204 (KN9204) and Jing 411 (J411). On a high-density genetic linkage map, 24, 26 and 25 QTL were associated with TKW, kernel length (KL), and kernel width (KW), respectively. A major and stable QTL, QTkw-2D, was mapped to an 8.3 cM interval on chromosome arm 2DL. By saturation of polymorphic markers in its target region, QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line (F6:7). This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines (NILs). NILsKN9204 had higher fresh and dry weights than NILsJ411 at various grain-filling stages. The TKW and KW of NILsKN9204 were much higher than those of NILsJ411 in field trials. By comparison of both DNA sequence and expression between KN9204 and J411, TraesCS2D02G460300.1 (TraesKN2D01HG49350) was assigned as a candidate gene for QTkw-2D. This was confirmed by RNA sequencing (RNA-seq) of QTkw-2D NILs. These results provide the basis of map-based cloning of QTkw-2D, and DNA markers linked to the candidate gene may be used in marker-assisted selection.

Keywords

Fine mappingCandidate geneWheatThousand-kernel weight

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The Crop Journal
Volume 11 Issue 5,
October 2023
Pages 1491-1500
DOI: 10.1016/j.cj.2023.03.007
Cite this article:
Meng D, Batool A, Xuan Y, et al. Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.). The Crop Journal, 2023, 11(5): 1491-1500. https://doi.org/10.1016/j.cj.2023.03.007

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Received: 14 December 2022
Revised: 16 February 2023
Accepted: 09 March 2023
Published: 11 April 2023
© 2023 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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