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Research paper | Open Access

Down-regulation of MeMYB2 leads to anthocyanin accumulation and increases chilling tolerance in cassava (Manihot esculenta Crantz)

Xin Guoa,b,c,d, Xiaohui Yue, Chenyu Line, Pingjuan Zhaoc,d, Bin Wangc,d, Liangping Zouc,d, Shuxia Lib,c,d,f, Xiaoling Yub,c, Yinhua Chene, Peng Zhangg, Ming Pengb,f( ), Mengbin Ruanb,c,d,f( )
College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China
Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, Hainan, China
College of Tropical Crops, Hainan University, Haikou 570228, Hainan, China
Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, Hainan, China
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
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Abstract

Chilling-induced accumulation of reactive oxygen species (ROS) is harmful to plants, which usually produce anthocyanins to scavenge ROS as protection from chilling stress. As a tropical crop, cassava is hypersensitive to chilling, but the biochemical basis of this hypersensitivity remains unclear. We previously generated MeMYB2-RNAi transgenic cassava with increased chilling tolerance. Here we report that MeMYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling stress. Under this stress, the anthocyanin biosynthesis pathway was more active in MeMYB2-RNAi lines than in the wild type, and several genes involved in the pathway, including MeTT8, were up-regulated by MeMYB2-RNAi in the transgenic cassava. MeMYB2 bound to the MeTT8 promoter and blocked its expression under both normal and chilling conditions, thereby inhibiting anthocyanin accumulation. MeTT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase (MeDFR-2) and increased MeDFR-2 expression. MeMYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.

Keywords

AnthocyaninsCassavaEarly chilling stressMeMYB2bHLH transcription factor

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The Crop Journal
Volume 11 Issue 4,
August 2023
Pages 1181-1191
DOI: 10.1016/j.cj.2023.03.009
Cite this article:
Guo X, Yu X, Lin C, et al. Down-regulation of MeMYB2 leads to anthocyanin accumulation and increases chilling tolerance in cassava (Manihot esculenta Crantz). The Crop Journal, 2023, 11(4): 1181-1191. https://doi.org/10.1016/j.cj.2023.03.009

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Received: 22 April 2022
Revised: 25 March 2023
Accepted: 28 March 2023
Published: 26 April 2023
© 2023 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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