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Research paper | Open Access

Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

Jie Lia,1, Amanpreet Kaurb,c,d,1, Brian Harrowerb, Miles Armstrongc, Daolong Doua,e, Xiaodan Wanga( ), Ingo Heina,b,c( )
Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China
The James Hutton Institute, Invergowrie, Dundee DD25DA, UK
University of Dundee, Dundee DD14HN, UK
Crop Research Centre, Teagasc, OakPark, Carlow R93 XE12, Ireland
College of Plant Protection, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

1 These authors contributed equally to this work.

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Abstract

More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

Keywords

Disease resistanceRenseqLate blightSolanum bulbocastanum

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The Crop Journal
Volume 11 Issue 6,
December 2023
Pages 1828-1835
DOI: 10.1016/j.cj.2023.08.005
Cite this article:
Li J, Kaur A, Harrower B, et al. Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum. The Crop Journal, 2023, 11(6): 1828-1835. https://doi.org/10.1016/j.cj.2023.08.005

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Received: 14 April 2023
Revised: 28 June 2023
Accepted: 17 September 2023
Published: 24 September 2023
© 2023 Crop Science Society of China and Institute of Crop Science, CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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