WPA1 encodes a vWA domain protein that regulates wheat plant architecture
Abstract
Plant height, spike, leaf, stem and grain morphologies are key components of plant architecture and related to wheat yield. A wheat (Triticum aestivum L.) mutant, wpa1, displaying temperature-dependent pleiotropic developmental anomalies, was isolated. The WPA1 gene, encoding a von Willebrand factor type A (vWA) domain protein, was located on chromosome arm 7DS and isolated by map-based cloning. The functionality of WPA1 was validated by multiple independent EMS-induced mutants and gene editing. Phylogenetic analysis revealed that WPA1 is monocotyledon-specific in higher plants. The identification of WPA1 provides opportunity to study the temperature regulated wheat development and grain yield.
References
Y. Wang, J. Li, Molecular basis of plant architecture, Annu. Rev. Plant Biol. 59 (2008) 253–279.
Y. Jiao, Y. Wang, D. Xue, J. Wang, M. Yan, G. Liu, G. Dong, D. Zeng, Z. Lu, X. Zhu, Q. Qian, J. Li, Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice, Nat. Genet. 42 (2010) 541–544.
Z. Lu, H. Yu, G. Xiong, J. Wang, Y. Jiao, G. Liu, Y. Jing, X. Meng, X. Hu, Q. Qian, X. Fu, Y. Wang, J. Li, Genome-wide binding analysis of the transcription activator ideal plant architecture1 reveals a complex network regulating rice plant architecture, Plant Cell 25 (2013) 3743–3759.
X. Song, Z. Lu, H. Yu, G. Shao, J. Xiong, X. Meng, Y. Jing, G. Liu, G. Xiong, J. Duan, X.F. Yao, C.M. Liu, H. Li, Y. Wang, J. Li, IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice, Cell Res. 27 (2017) 1128–1141.
J. Wang, L. Zhou, H. Shi, M. Chern, H. Yu, H. Yi, M. He, J. Yin, X. Zhu, Y. Li, W. Li, J. Liu, J. Wang, X. Chen, H. Qing, Y. Wang, G. Liu, W. Wang, P. Li, X. Wu, L. Zhu, J.M. Zhou, P.C. Ronald, S. Li, J. Li, X. Chen, A single transcription factor promotes both yield and immunity in rice, Science 361 (2018) 1026–1028.
L. Zhang, H. Yu, B. Ma, G. Liu, J. Wang, J. Wang, R. Gao, J. Li, J. Liu, J. Xu, Y. Zhang, Q. Li, X. Huang, J. Xu, J. Li, Q. Qian, B. Han, Z. He, J. Li, A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice, Nat. Commun. 8 (2017) 14789.
L.E. Dixon, M. Pasquariello, R. Badgami, K.A. Levin, G. Poschet, P.Q. Ng, S. Orford, N. Chayut, N.M. Adamski, J. Brinton, J. Simmonds, B. Steuernagel, I.R. Searle, C. Uauy, S.A. Boden, MicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat, Sci. Adv. 8 (2022) eabn5907.
X. Zhang, H. Jia, T. Li, J. Wu, R. Nagarajan, L. Lei, C. Powers, C.C. Kan, W. Hua, Z. Liu, C. Chen, B.F. Carver, L. Yan, TaCol-B5 modifies spike architecture and enhances grain yield in wheat, Science 376 (2022) 180–183.
C.A. Whittaker, R.O. Hynes, Distribution and evolution of von Willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere, Mol. Biol. Cell 13 (2002) 3369–3387.
Q. Wang, Y. Chen, S. Li, W. Yang, L. Sun, M. Jang, X. Wu, Q. Wang, L. Chen, Y. Wu, Ca2+-based allosteric switches and shape shifting in RGLG1 VWA domain, Comput. Struct. Biotechnol. J. 18 (2020) 821–833.
Q. Wang, M. Jiang, M.N. Isupov, Y. Chen, J.A. Littlechild, L. Sun, X. Wu, Q. Wang, W. Yang, L. Chen, Q. Li, Y. Wu, The crystal structure of Arabidopsis BON1 provides insights into the copine protein family, Plant J. 103 (2020) 1215–1232.
Y. Wang, M. Abrouk, S. Gourdoupis, D.H. Koo, M. Karafiátová, I. Molnár, K. Holušová, J. Doležel, N. Athiyannan, E. Cavalet-Giorsa, L. Jaremko, J. Poland, S.G. Krattinger, An unusual tandem kinase fusion protein confers leaf rust resistance in wheat, Nat. Genet. 55 (2023) 914–920.
C.N. Jacott, S.A. Boden, Feeling the heat: developmental and molecular responses of wheat and barley to high ambient temperatures, J. Exp. Bot. 71 (2020) 5740–5751.
M. Ejaz, M. von Korff, The genetic control of reproductive development under high ambient temperature, Plant Physiol. 173 (2017) 294–306.
G. Li, H.N.J. Kuijer, X. Yang, H. Liu, C. Shen, J. Shi, N. Betts, M.R. Tucker, W. Liang, R. Waugh, R.A. Burton, D. Zhang, MADS1 maintains barley spike morphology at high ambient temperatures, Nat. Plants 7 (2021) 1093–1107.
Iwgsc, Shifting the limits in wheat research and breeding using a fully annotated reference genome, Science 361 (2018) eaar7191.
P. Lu, L. Guo, Z. Wang, B. Li, J. Li, Y. Li, D. Qiu, W. Shi, L. Yang, N. Wang, G. Guo, J. Xie, Q. Wu, Y. Chen, M. Li, H. Zhang, L. Dong, P. Zhang, K. Zhu, D. Yu, Y. Zhang, K. R. Deal, N. Huo, C. Liu, M.C. Luo, J. Dvorak, Y.Q. Gu, H. Li, Z. Liu, A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew, Nat. Commun. 11 (2020) 680.
G.C. Allen, M.A. Flores-Vergara, S. Krasynanski, S. Kumar, W.F. Thompson, A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide, Nat. Protoc. 1 (2006) 2320–2325.
R.W. Michelmore, I. Paran, R.V. Kesseli, Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations, Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 9828–9832.
F.M. You, N. Huo, Y.Q. Gu, M.C. Luo, Y. Ma, D. Hane, G.R. Lazo, J. Dvorak, O.D. Anderson, BatchPrimer3: a high throughput web application for PCR and sequencing primer design, BMC Bioinformatics 9 (2008) 253.
S. Ma, M. Wang, J. Wu, W. Guo, Y. Chen, G. Li, Y. Wang, W. Shi, G. Xia, D. Fu, Z. Kang, F. Ni, WheatOmics: a platform combining multiple omics data to accelerate functional genomics studies in wheat, Mol. Plant 14 (2021) 1965–1968.
E. Lander, P. Green, J. Abrahamson, A. Barlow, M. Daly, S. Lincoln, L. Newberg, L. Newburg, MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations, Genomics 1 (1987) 174–181.
R.H. Liu, J.L. Meng, MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data, Yi Chuan 25 (2003) 317–321.
Y. Ishida, M. Tsunashima, Y. Hiei, T. Komari, Wheat (Triticum aestivum L.) transformation using immature embryos, Methods Mol. Biol. 1223 (2015) 189–198.
S.D. Yoo, Y.H. Cho, J. Sheen, Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis, Nat. Protoc. 2 (2007) 1565–1572.
N.L. Bray, H. Pimentel, P. Melsted, L. Pachter, Near-optimal probabilistic RNA-seq quantification, Nat. Biotechnol. 34 (2016) 525–527.
D. Kim, J.M. Paggi, C. Park, C. Bennett, S.L. Salzberg, Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype, Nat. Biotechnol. 37 (2019) 907–915.
M.I. Love, W. Huber, S. Anders, Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2, Genome Biol. 15 (2014) 550.
Y. Zhou, X. Zhao, Y. Li, J. Xu, A. Bi, L. Kang, D. Xu, H. Chen, Y. Wang, Y.G. Wang, S. Liu, C. Jiao, H. Lu, J. Wang, C. Yin, Y. Jiao, F. Lu, Triticum population sequencing provides insights into wheat adaptation, Nat. Genet. 52 (2020) 1412–1422.
X. Zhao, Y. Guo, L. Kang, C. Yin, A. Bi, D. Xu, Z. Zhang, J. Zhang, X. Yang, J. Xu, S. Xu, X. Song, M. Zhang, Y. Li, P. Kear, J. Wang, Z. Liu, X. Fu, F. Lu, Population genomics unravels the Holocene history of bread wheat and its relatives, Nat. Plants 9 (2023) 403–419.
M. Quint, C. Delker, K.A. Franklin, P.A. Wigge, K.J. Halliday, M. van Zanten, Molecular and genetic control of plant thermomorphogenesis, Nat. Plants 2 (2016) 15190.
J. Liu, N. Jambunathan, T.W. McNellis, Transgenic expression of the von Willebrand A domain of the BONZAI 1/COPINE 1 protein triggers a lesion-mimic phenotype in Arabidopsis, Planta 221 (2005) 85–94.
T. Jing, Y. Wu, Y. Yu, J. Li, X. Mu, L. Xu, X. Wang, G. Qi, J. Tang, D. Wang, S. Yang, J. Hua, M. Gou, Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis, Nat. Commun. 15 (2024) 2028.
S. Thangasamy, P.W. Chen, M.H. Lai, J. Chen, G.Y. Jauh, Rice LGD1 containing RNA binding activity affects growth and development through alternative promoters, Plant J. 71 (2012) 288–302.
D. Tuckwell, Evolution of von Willebrand factor A (VWA) domains, Biochem. Soc. Trans. 27 (1999) 835–840.
J.O. Lee, P. Rieu, M.A. Arnaout, R. Liddington, Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18), Cell 80 (1995) 631–638.
京公网安备11010802044758号