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Research Article | Open Access

In vitro modulation of TH1 and TH2 cytokine expression by edible tuber of Dioscorea alata and study of correlation patterns of the cytokine expression

Priyankar Dey, Tapas Kumar Chaudhuri( )
Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri 734013, West Bengal, India

Peer review under responsibility of Beijing Academy of Food Sciences.

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Abstract

The underground tuber of Dioscorea alata is a palatable food and is also claimed to have therapeutic effects. Various groups of researchers demonstrated different activities of this tuber such as antioxidant, hepatoprotective, anti-ulcer and anti-diabetic activities. Therefore, the aim of the present study was to evaluate the immunomodulatory potentialities of 70% hydro-methanolic extract of D. alata underground tubers by investigating different parameters like the expression of interferon gamma (IFN-γ), interleukin (IL)-2, IL-4 and IL-10, in addition to its effect on the proliferation of murine splenic T-lymphocytes in vitro. Results demonstrated that D. alata can actively polarize the TH0 lymphocyte population towards the expression of TH1 immune response by up-regulating the IFN-γ and IL-2 expression and down-regulating the IL-4 and IL-10 expression. The tuber extract also proved to possess mitogenic activity as evidenced by the proliferation of lymphocytes in vitro.

Keywords

ProliferationImmunomodulationAnti-inflammatoryCytokineMTTDioscorea (yam)Lymphocyte

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Food Science and Human Wellness
Volume 3 Issue 1,
March 2014
Pages 1-8
DOI: 10.1016/j.fshw.2014.01.001
Cite this article:
Dey P, Chaudhuri TK. In vitro modulation of TH1 and TH2 cytokine expression by edible tuber of Dioscorea alata and study of correlation patterns of the cytokine expression. Food Science and Human Wellness, 2014, 3(1): 1-8. https://doi.org/10.1016/j.fshw.2014.01.001

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Received: 20 November 2013
Revised: 03 January 2014
Accepted: 17 January 2014
Published: 27 January 2014
© 2014 Beijing Academy of Food Sciences.
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