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Research Article | Open Access

Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method

Zhongqiang Chen1, Ke Zhang1, Huan Yin, Qi Li, Lan Wang, Zhiguo Liu( )
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China

1 These authors contributed equally to this work.Peer review under responsibility of Beijing Academy of Food Sciences.]]>

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Abstract

Salmonella Choleraesuis, S. Enteritidis and S. Typhimurium are the main pathogens that contaminate animal products and cause human Salmonella food poisoning. To establish the loop-mediated isothermal amplification (LAMP) method for the rapid detection of Salmonella and 3 common Salmonella serotypes, inner and outer primer sets targeting Salmonella invE gene and 3 serotype-specific genes fliC, lygD and STM4495 were designed. The LAMP reaction conditions were optimized. The specificity of LAMP primers was identified by testing 10 different bacterial strains including S. Choleraesuis, S. Enteritidis and S. Typhimurium. Take S. Choleraesuis as example, the detection limit of LAMP assay was 1.33×101 CFU/mL for bacteria culture and 2.0×101 CFU/mL for simulated pork sample. The results show that LAMP is a rapid, sensitive and specific method for Salmonella detection and can be used for the rapid detection of Salmonella in food.

Keywords

DetectionLoop-mediated isothermal amplificationSalmonella EnteritidisSalmonellaSalmonella CholeraesuisSalmonella Typhimurium

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Food Science and Human Wellness
Volume 4 Issue 2,
June 2015
Pages 75-79
DOI: 10.1016/j.fshw.2015.05.001
Cite this article:
Chen Z, Zhang K, Yin H, et al. Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method. Food Science and Human Wellness, 2015, 4(2): 75-79. https://doi.org/10.1016/j.fshw.2015.05.001

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Received: 01 March 2015
Revised: 08 May 2015
Accepted: 15 May 2015
Published: 26 May 2015
© 2015 Beijing Academy of Food Sciences.
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