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Research Article | Open Access

Phenolics extract of Tetrapleura tetraptera fruit inhibits xanthine oxidase and Fe2+-induced lipid peroxidation in the kidney, liver, and lungs tissues of rats in vitro

Emmanuel Anyachukwu Irondia( ), Ganiyu Obohb, Samson Olalekan Agboolac, Aline Augusti Boligond, Margareth Linde Athayded
Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, P.M.B. 1530, Ilorin, Nigeria
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria, P.M.B. 704, Akure 340001, Nigeria
Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Nigeria
Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Building 26, Room 1115, Santa Maria CEP 97105-900, Brazil

Peer review under responsibility of Beijing Academy of Food Sciences.

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Abstract

The phenolics composition and inhibitory activity of Tetrapleura tetraptera fruit extract on xanthine oxidase (XO) and Fe2+-induced lipid peroxidation in the kidney, liver and lungs tissues of rats were evaluated in vitro. Phenolics (flavonoids and phenolic acids) were quantified using reverse-phase high performance liquid chromatrography coupled with diode array detection (HPLC-DAD). The XO and Fe2+-induced lipid peroxidation inhibitory abilities of the extract were evaluated using spectrophotometric methods. The extract contained some flavonoids and phenolic acids, including catechin, epicatechin, rutin, quercetin, luteolin, apigenin; gallic, chlorogenic, caffeic and ellagic acids that are beneficial to health. The extract inhibited XO in the kidney, liver and lungs tissues in a dose-dependent manner. The half-maximal inhibitory concentrations (IC50) of the extract on XO from the tissues varied significantly (P < 0.05), and were in the order of liver>kidney>lungs. The extract also inhibited Fe2+-induced lipid peroxidation in a dose-dependent pattern, having IC50 in the order of liver > lungs > kidney. T. tetraptera fruit extract could be a promising nutraceutical for preventing and managing hyperuricaemia and the associated disease conditions, due to its ability to inhibit XO; this bioactivity is attributable to combined effect of its flavonoids and phenolic acids.

Keywords

Lipid peroxidationPhenolicsXanthine oxidaseTetrapleura tetrapteraHyperuricaemia

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Food Science and Human Wellness
Volume 5 Issue 1,
March 2016
Pages 17-23
DOI: 10.1016/j.fshw.2015.11.001
Cite this article:
Irondi EA, Oboh G, Agboola SO, et al. Phenolics extract of Tetrapleura tetraptera fruit inhibits xanthine oxidase and Fe2+-induced lipid peroxidation in the kidney, liver, and lungs tissues of rats in vitro. Food Science and Human Wellness, 2016, 5(1): 17-23. https://doi.org/10.1016/j.fshw.2015.11.001

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Received: 02 September 2015
Revised: 04 November 2015
Accepted: 06 November 2015
Published: 17 November 2015
© 2015 Beijing Academy of Food Sciences.
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