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Review Article | Open Access

PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources

Jin-Qiang Chena( ), Stephanie Healeya, Patrick Regana, Pongpan Laksanalamaib, Zonglin Hua( )
Winchester Engineering & Analytical Center (WEAC), HFR-NE460, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton Street, Winchester, MA 01890, USA
MD DHMH Laboratories Administration, Division of Microbiology, Room 528, 1770 Ashland Ave. Baltimore, MD 21205, USA

Peer review under responsibility of Beijing Academy of Food Sciences.

Show Author Information

Abstract

Listeria monocytogenes is an important foodborne pathogen responsible for listeriosis, a fatal disease. It is widely distributed in various foods and environmental sources. In this review, we focused on addressing PCR-based technologies, including conventional PCR, qPCR and droplet digital PCR (ddPCR). Specifically, we described (a) conventional PCR and mono-, duplex- and multiplex-qPCR methodologies; (b) development and applications of gene HlyA-, Iap-, PrfA – and SsrA-based conventional and qPCR assays as well as PCR assays targeting newly identified gene targets for specific detection of L. monocytogenes; differentiation of viable from dead L. monocytogenes by qPCR in conjugation with propidium monoazide pretreatment; PCR-based serotype identification of L. monocytogenes isolates; PCR-based detection of L. ivanovii, infecting ruminants, differentiation of L. monocytogenes from other Listeria species; and sigB-gene based PCR identification of Listeria spp; (c) applications of ddPCR in detection of L. monocytogenes; and (d) application of qPCR assays in detection and subtyping of L. monocytogenes in milk and dairy products; meats, meat products and meat-processing environment; and seafood, seafood products and processing environment. Our goal was to provide a relatively comprehensive overview of PCR-based methodologies available in detection, characterization and subtyping of various strains of L. monocytogenes in foods and environmental sources.

Keywords

Foodborne pathogensListeria monocytogenesListeria ivanoviiqPCR

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Food Science and Human Wellness
Volume 6 Issue 2,
June 2017
Pages 39-59
DOI: 10.1016/j.fshw.2017.03.001
Cite this article:
Chen J-Q, Healey S, Regan P, et al. PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources. Food Science and Human Wellness, 2017, 6(2): 39-59. https://doi.org/10.1016/j.fshw.2017.03.001

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Received: 01 February 2017
Revised: 24 March 2017
Accepted: 30 March 2017
Published: 02 April 2017
© 2017 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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