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Research Article | Open Access

Interaction mechanism of egg white- derived ACE inhibitory peptide TNGIIR with ACE and its effect on the expression of ACE and AT1 receptor

Zhipeng Yua,b, Hui Guoa, David Shiuana, Chensi Xiaa, Wenzhu Zhaoa( ), Long Dingc, Fuping Zhengb( ), Jingbo Liuc( )
College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 102488, PR China
Lab of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

The egg white-derived hexapeptide TNGIIR inhibits angiotensin-converting enzyme (ACE) activity in vitro. In this work, molecular docking revealed that TNGIIR established hydrogen bonds with the S1 (Ala 354), S2 (Gln 281, His 513, Tyr 520 and Lys 511) and S1′ (Glu 162) pockets of ACE. In addition, the potential antihypertensive effect of the oral administration of TNGIIR in spontaneously hypertensive rats (SHR) was investigated, as was the effect of this peptide on the mRNA expression of ACE and angiotensin type 1 (AT1) and type 2 (AT2) receptors in renal tissue. The oral administration of TNGIIR (2, 10 and 50 mg/kg) for up to four weeks did not reduce the blood pressure of SHR, in contrast to captopril (10 mg/kg, orally), but attenuated the mRNA expression of ACE and AT1 receptor (as did captopril). In contrast, both TNGIIR and captopril enhanced the expression of AT2 receptor mRNA. There was no change in the circulating concentration of fAng Ⅰ, 10 amino acids, but a slight decrease (about 10%) was seen in the concentration of circulating angiotensin Ⅱ with TNGIIR and captopril.

Keywords

Molecular dockingGene expressionACE inhibitory peptideEgg white proteinsAntihypertensive effect

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Food Science and Human Wellness
Volume 9 Issue 1,
March 2020
Pages 52-57
DOI: 10.1016/j.fshw.2019.12.009
Cite this article:
Yu Z, Guo H, Shiuan D, et al. Interaction mechanism of egg white- derived ACE inhibitory peptide TNGIIR with ACE and its effect on the expression of ACE and AT1 receptor. Food Science and Human Wellness, 2020, 9(1): 52-57. https://doi.org/10.1016/j.fshw.2019.12.009

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Received: 22 September 2019
Revised: 16 December 2019
Accepted: 23 December 2019
Published: 24 December 2019
© 2020 "Society information".

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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