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Research Article | Open Access

Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathways

Jie Lina, Jinlong Tiana,b, Chi Shua,b, Zhen Chenga, Yunen Liuc, Weisheng Wangd, Ruihai Liue, Bin Lia,b,c( ), Yuehua Wanga,b,c( )
National R & D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR China
Key Laborotary of Healthy Food Nutrition and Innovative Manufacturing, Liaoning, PR China
Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Trauma PLA, Liaoning, PR China
Shenyang Blueberry Industry co. LTD., Liaoning, PR China
Department of Food Science, Cornell University, Ithaca, NY, USA

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Malvidin-3-galactoside (Mv-3-gal) is the major anthocyanin monomer in blueberry anthocyanins. The compound is well-characterized by its anti-oncogenesis function in multiple organs including liver. In the current study, the mechanism driving the anti-hepatocellular carcinoma (HCC) function of Mv-3-gal was further explored by focusing on apoptosis and metastases pathways. HCC cell line Huh-7 was firstly administrated with Mv-3-gal of different concentrations and the effect of Mv-3-gal on cell proliferation, apoptosis, colony formation ability, metastasis potential as well as the activity of Akt/PTEN and MAPK pathways was assessed. The results showed that Mv-3-gal inhibited the cell proliferation and colony formation ability, induced cell cycle arrest and apoptosis in a dose-dependent manner. Regarding the metastasis potential, Mv-3-gal suppressed the migration and invasion potential of Huh-7 cells by regulating MMPs expression. Taken together, the findings highlighted the anti-HCC potent of Mv-3-gal, which was associated with the inhibition of Akt/PTEN, MAPK and MMP pathways.

Keywords

ApoptosisHepatocellular carcinomaMalvidin-3-galactosideMAPK signaling

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Food Science and Human Wellness
Volume 9 Issue 2,
June 2020
Pages 136-145
DOI: 10.1016/j.fshw.2020.02.004
Cite this article:
Lin J, Tian J, Shu C, et al. Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathways. Food Science and Human Wellness, 2020, 9(2): 136-145. https://doi.org/10.1016/j.fshw.2020.02.004

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Received: 04 November 2019
Revised: 03 February 2020
Accepted: 14 February 2020
Published: 15 February 2020
© 2020 "Society information". Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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