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Research Article | Open Access

Identification and molecular mechanism of angiotensin-converting enzyme inhibitory peptides from Larimichthys crocea titin

Yue Fana, Zhipeng Yua,b( ), Wenzhu Zhaoa, Long Dingc, Fuping Zhengb, Jianrong Lib, Jingbo Liud
College of Food Science and Engineering, Bohai University, Jinzhou, 121013, China
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 102488, China
College of Food Science and Engineering, Northwest A & F University, Yangling, 712100, China
Lab of Nutrition and Functional Food, Jilin University, Changchun, 130062, China

Peer review under responsibility of KeAi Communications Co., Ltd

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Abstract

This study aimed to identify novel ACEI peptides from Larimichthys crocea titin using in silico approaches and to clarify the molecular interaction mechanism. The hydrolyzed peptides of titin were compared with known ACEI peptides in the AHTPDB and BIOPEP-UWM database. Furthermore, peptides were evaluated for their solubility, ADMET properties, △G (kcal/mol) values, and in vitro ACEI activity. Molecular mechanism of ACE-peptide was performed by molecular interactions and binding orientation study. The results revealed that IC50 values of Trp-Ala-Arg(WAR) and Trp-Gln-Arg(WQR) were (31.2 ± 0.8) and (231.33 ± 0.02) μmol/L, respectively. The docking interactions result suggested that ACE-WAR and ACE-WQR complexes have same binding site, including the residues LYS511, TYR520, TYR523, HIS353, and HIS513. Molecular docking of two tripeptides WAR and WQR with ACE studies predicted their binding site and clarified the interaction between ACE and its inhibitors. The molecular docking data are consistent with the ACE inhibitory activity of the studied peptides. The results showed that Larimichthys crocea titin may be a valuable source for developing nutraceutical food.

Keywords

Molecular dockingIdentificationACE peptideIn silico approachesADMET

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Food Science and Human Wellness
Volume 9 Issue 3,
September 2020
Pages 257-263
DOI: 10.1016/j.fshw.2020.04.001
Cite this article:
Fan Y, Yu Z, Zhao W, et al. Identification and molecular mechanism of angiotensin-converting enzyme inhibitory peptides from Larimichthys crocea titin. Food Science and Human Wellness, 2020, 9(3): 257-263. https://doi.org/10.1016/j.fshw.2020.04.001

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Received: 09 October 2019
Revised: 02 February 2020
Accepted: 03 April 2020
Published: 23 April 2020
© 2020 "Society information". Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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