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Research Article | Open Access

Development of water-soluble zein colloid particles and in situ antibacterial evaluation by multiple headspace extraction gas chromatography

Yan Yina,c( ), Fuzhen Zhoub, Yechong Yinb, Yonghong Penga,c( )
School of Life Science, Huizhou University, Huizhou 516007, China
Research and Development Center of Food Proteins, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
School of Life Science, South China Normal University, Guangzhou 510640, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

In this work, water-soluble zein/sodium caseinate (SC) nanoparticles were prepared as a delivery system of antimicrobials through anti-solvent procedure. Two model antimicrobials (nisin and thymol) were coencapsulated into zein particles by different interaction. Release properties indicate that zein colloid particles can be used not only in neutral food but also in acidic foods. It is worth mention that the release of thymol was delayed simply by adding nisin. To investigate the antimicrobial properties of zein/SC particles, Escherichia coli and Bacillus subtilis, Listeria monocytogenes were selected as gram-negative and gram-positive indicator bacteria. CO2 produced by incubated germs was detected by headspace gas chromatography. Long-term effective antimicrobial activities of zein particles in Luria broth was attained by burst release of nisin and slowly sustained release of thymol. Synergistic antimicromibial properties against L. monocytogenes of thymol and nisin loaded by zein/SC particles in milk were also observed. The antimicrobial property of zein/SC particles could enhance microbial safety of food, giving this delivery system promising applications in food industry.

Keywords

AntimicrobialZein/SC nanoparticlesThymolNisinDelivery system

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Food Science and Human Wellness
Volume 10 Issue 2,
March 2021
Pages 191-196
DOI: 10.1016/j.fshw.2021.02.008
Cite this article:
Yin Y, Zhou F, Yin Y, et al. Development of water-soluble zein colloid particles and in situ antibacterial evaluation by multiple headspace extraction gas chromatography. Food Science and Human Wellness, 2021, 10(2): 191-196. https://doi.org/10.1016/j.fshw.2021.02.008

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Received: 04 November 2019
Revised: 07 February 2020
Accepted: 13 August 2020
Published: 22 March 2021
© 2021 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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