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Research Article | Open Access

Effects of common prebiotics on iron status and production of colonic short-chain fatty acids in anemic rats

Fan Zhanga, Ken K.L. Yungb, Chi KongYeungc( )
Division of Science and Technology, BNU-HKBU United International College, Zhuhai, Guangdong, China
Department of Biology, Hong Kong Baptist University, Hong Kong, China
Animal Science Department, California Polytechnic State University, San Luis Obispo, California, USA

Peer review under responsibility of KeAi Communications Co., Ltd

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Abstract

Prebiotics may enhance iron absorption, and one plausible mechanism involves the production of short-chain fatty acids (SCFA) in the colon by intestinal microflora. The objectives of this study were to determine the effects of common commercially-available prebiotics including fructooligosaccharide (FOS), inulin, FOS-inulin mixture, galactooligosaccharide (GOS), and lactulose on the iron status of anemic rats, and to monitor changes in the production of colonic SCFA. Anemic Sprague-Dawley rats receiving a low-iron diet (12μg Fe/g diet) were supplemented with or without prebiotics (5% m/V in drinking water) for 5 weeks. Hemoglobin concentration in rats supplemented with GOS after 3 weeks (4.3g/dL) was significantly higher than rats without supplementation (3.7g/dL), while FOS also significantly increased hemoglobin concentration after 4 weeks (4.1g/dL vs. 3.7g/dL). All other prebiotics showed no effects. Anemic rats showed lower overall SCFA production in the colon than normal rats, and only FOS significantly increased the production of the three main SCFA (acetic acid, propionic acid and isobutyric acid) identified in anemic rats, with other prebiotics showing no noticeable trends. Our results suggest that GOS and FOS may slightly improve iron status of anemic rats, but the role of SCFA in the colon is not clear.

Keywords

IronShort-chain fatty acidsNon-digestible oligosaccharidesPrebioticsRat

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Food Science and Human Wellness
Volume 10 Issue 3,
May 2021
Pages 327-334
DOI: 10.1016/j.fshw.2021.02.024
Cite this article:
Zhang F, Yung KK, KongYeung C. Effects of common prebiotics on iron status and production of colonic short-chain fatty acids in anemic rats. Food Science and Human Wellness, 2021, 10(3): 327-334. https://doi.org/10.1016/j.fshw.2021.02.024

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Received: 05 August 2020
Revised: 22 September 2020
Accepted: 08 October 2020
Published: 16 April 2021
© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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