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Research Article | Open Access

Anti-colon cancer activity of water-soluble polysaccharides extracted from Gloeostereum incarnatum via Wnt/β-catenin signaling pathway

Jiawei Hea,b, Anhui Yangb, Xuyu Zhaoa, Yang Liua, Shuyan Liua( ), Di Wangb( )
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China
School of Life Sciences, Jilin University, Changchun 130012, China

Peer review under responsibility of KeAi Communications Co., Ltd

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Abstract

The incidence rate of colon cancer ranks the third among malignant tumors worldwide. Gloeostereum incarnatum (GI), a fungus with homology of medicine and food, has multiple pharmacological activities. It was investigated in this study that the anti-colon cancer effect of polysaccharides purified from GI (GIPS) in ApcMinC/Gpt mice (an in situ colon cancer mouse model). Eight-week administration with GIPS at doses of 30 or 90mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without influencing the animals' body weight and organ functions. According to the proteomics performing by antibody array, among 308 detected cytokines, GIPS significantly regulated 89 of them. Compared with vehicle-treated mice, GIPS effectively reduced the levels of interleukin (IL)-1β, IL-4, IL-6, IL-17, IL-22, tumor necrosis factor (TNF)-α, matrix metalloproteinase (MMP)-2, and enhanced the levels of IL-15 and IL-18 in serum and/or colon tissues, which suggested its anti-inflammation of GIPS. GIPS suppressed nuclear aggregation of β-catenin, affected the expression of WNT1 and related proteins, thereby regulated the activation of the Wnt signaling. Altogether, GIPS can inhibit the growth of colon cancer, at least partially, via inhibiting the Wnt/β-catenin signaling pathway.

Keywords

Gloeostereum incarnatumWater-soluble polysaccharides, Colon cancerWnt/β-catenin

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Food Science and Human Wellness
Volume 10 Issue 4,
July 2021
Pages 460-470
DOI: 10.1016/j.fshw.2021.04.008
Cite this article:
He J, Yang A, Zhao X, et al. Anti-colon cancer activity of water-soluble polysaccharides extracted from Gloeostereum incarnatum via Wnt/β-catenin signaling pathway. Food Science and Human Wellness, 2021, 10(4): 460-470. https://doi.org/10.1016/j.fshw.2021.04.008

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Received: 14 April 2020
Revised: 23 May 2020
Accepted: 27 May 2020
Published: 04 June 2021
© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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