AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Food Science and Human Wellness Article
PDF (339.4 KB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

The normal cell proliferation and wound healing effect of polysaccharides from Ganoderma amboinense

Shuangzhi Zhaoa,b, Ming Leic, Hui Xua, Hailun Hec, Alexander Suvorova, Junhua Wanga, Jiying Qiua, Qingxin Zhoua, Jinyu Yanga( ), Leilei Chena( )
Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Jinan 250100, China
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
School of Life Science, Central South University, Changsha 410013, China

Peer review under responsibility of KeAi Communications Co., Ltd

Show Author Information

Abstract

To study the cell proliferation and wound healing activity of polysaccharides from Ganoderma amboinense (GAMPS), the polysaccharide was extracted by water extraction and alcohol precipitation method, and its monosaccharide composition and molecular weight were analyzed. The effects of different concentrations of GAMPS on the cell proliferation were determined by cell survival rate test, and the wound healing ability of GAMPS to NIH/3T3 cells was detected. The preliminary evaluation of the antioxidant ability of GAMPS was conducted by the oxygen radical absorbance capacity (ORAC). The results showed that the GAMPS was composed of glucose, mannose, and galactose at a molar ratio of 67.62:14.07:7.50, and the weight-average molecular weights were 5.439×106 and 1.704×105 g/mol by using high-performance gel-permeation chromatography-multiple angle laser scatter (HPGPC-MALS) analysis. GAMPS (0.2 μg/μL) showed the strongest proliferation ability to THP-1 cells, with cell survival rate of 178.7%. The wound healing effect of GAMPS (0.1 μg/μL) was obvious on NIH/3T3 and 3.75 μg/μL of GAMPS showed the strongest total antioxidant ability. All the results indicate that GAMPS promotes cell proliferation, and has cell wound healing effect and strong antioxidant activity. The results provide theoretical foundation for the development and utilization of GAMPS.

Keywords

Wound healingAntioxidant activityGanoderma amboinense mycelium polysaccharideProliferation promotion

References

[1]

R.Y. Chen, Review of research on chemical constituents of Ganoderma lucidum and its quality control method, Edible and Medicinal Mushrooms 23 (5) (2015) 270-275
Google Scholar
[2]

R.Y. Tao, L.M. Hao, Q. Chen, et al., Recent progress in pharmacological activity of polysaccharides from the mycelia of liquid-cultured Ganoderma lucidum, Food Sci. 36 (9) (2015) 260-264
Google Scholar
[3]

C.F.R.I. Ferreira, S.A. Heleno, F.S. Reis, et al., Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities, Phytochemistry 114 (2015) 38-55. https://doi.org/10.1016/j.phytochem.2014.10.011
Crossref Google Scholar
[4]

S.S. Ferreira, C.P. Passos, P. Madureira, et al., Structure-function relationships of immunostimulatory polysaccharides: a review, Carbohyd. Polym. 132 (2015) 378-396. https://doi.org/10.1016/j.carbpol.2015.05.079
Crossref Google Scholar
[5]

L.X. Zhang, Y.J. Zhang, L.P. Zhang, Extraction and purification of polysaccharide from Ganoderma lucidum and its immunological activities, Journal of Northwest A & F University (Natural Science Edition) 42 (6) (2014) 168-172
Google Scholar
[6]

J.Z. He, P. Shao, H.D. Ni, et al., Study on the structure and constituents of polysaccharide from Ganoderma lucidum, Spectrosc. Spect. Anal. 30 (1) (2010) 123-127
Google Scholar
[7]

J.Q. Wang, S.P. Nie, Q. Yu, et al., Immune modulation and antioxidation activity of polysaccharides from Ganoderma atrum in immunosuppressed mice, Food Sci. 33 (23) (2012) 274-276. https://doi.org/10.35371/kjoem.2012.24.3.274
Crossref Google Scholar
[8]

L.J. Zhang, L. Zhang, The study of Ganoderma licidum polysaccharide combined cisplatin on A549 lung cancer cell proliferation inhibition, Modern Preventive Medicine 43 (9) (2016) 1670-1674
Google Scholar
[9]

Z.J. Zhang, S.F. Li, X.S. Wei, et al., Study on antioxidant activity of Ganoderma lucidum polysaccharide, Chem. Bioeng. 28 (3) (2011) 63-65
Google Scholar
[10]

J. Mao, H.L. Ma, Research progress of Ganoderma lucidum polysaccharides, Food Sci. 31 (1) (2010) 295-299
Google Scholar
[11]

J.G. Jiang, A. Hirakawa, B.S. Min, et al., In vivo antitumor effects of bitter principles from the antlered form of fruiting bodies of Ganoderma lucidum, J. Nat. Med. - Tokyo 60 (1) (2006) 42-48. https://doi.org/10.1007/s11418-005-0003-5
Crossref Google Scholar
[12]

L.J. Yang, Y.H. You, Z.B. Lin, et al., Protective effects of Ganoderma lucidum polysaccharides peptide on human umbilical vein endothelial cells injury by reactive oxygen species, Chinese Pharmacological Bulletin 26 (5) (2010) 657-660
Google Scholar
[13]

P.R. Song, J.D. Chang, Screening of Ganoderma lucidum strains with high extracellular polysaccharide productivity and the optimization of its submerged culture medium, Acta Edulis Fungi 10 (2) (2003) 9-16
Google Scholar
[14]

J.G. Liang, Y.Y. Chen, Y.L. Fang, et al., Study on the optimization and fermentation process of Gendarme lucidum extrapolysaccharide, Journal of Changshu Institute of Technology 19 (2) (2005) 71-76
Google Scholar
[15]

H.S. Pei, J.S. Sun, M.J. Wang, et al., Effect of lignin on growth of Ganoderma lucidum, Nongye Gongcheng Xuebao 33 (6) (2017) 309-314
Google Scholar
[16]

H. Xu, S.Z. Zhang, X.Y. Liu, et al., Optimization of liquid fermentation technology for intracellular polysaccharide from Ganoderma lucidum by response surface methodology, Jiangsu Agricultural Sciences 46 (23) (2018) 215-220
Google Scholar
[17]

J.Y. Yang, Y.H. Zhang, S.Z. Zhao, et al., Statistical optimization of medium for pullulan production by Aureobasidium pullulans NCPS2016 using fructose and soybean meal hydrolysates, Molecules 23 (6) (2018) 1334. https://doi.org/10.3390/molecules23061334
Crossref Google Scholar
[18]

A. Dávalos, C. Gómez-Cordovés, B.A. Bartolomé, Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay, J. Agric. Food Chem. 52 (1) (2004) 48-54. https://doi.org/10.1021/jf0305231
Crossref Google Scholar
[19]

M. Shi, Z.Y. Zhang, Y.N. Yang, Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP), Carbohyd. Polym. 95 (1) (2013) 200-206. https://doi.org/10.1016/j.carbpol.2013.02.081
Crossref Google Scholar
[20]

L.S. Ma, H.X. Chen, W.C. Zhu, et al., Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted from mushroom Inonotus obliquus, Food Res. Int. 50 (2) (2013) 633-640. https://doi.org/10.1016/j.foodres.2011.05.005
Crossref Google Scholar
[21]

H.Y. Wang, J. Dai, S.W. Chen, Separetion, monosaccharide composition and molecular weight analysis of polysaccharide from G. lucidum mycelium, Food and Machinery 31 (5) (2015) 201-205. https://doi.org/10.13652/j.issn.1003-5788.2015.05.052
Crossref Google Scholar
[22]

J. Si, G. Meng, Y. Wu, et al., Medium composition optimization, structural characterization, and antioxidant activity of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi, Int. J. Biol. Macromol. 124 (2019) 1186-1196. https://doi.org/10.1016/j.ijbiomac.2018.11.274
Crossref Google Scholar
[23]

G.M. Ren, L.M. Xu, T.Y. Lu, et al., Structural characterization and antiviral activity of lentinan from Lentinus edodes mycelia against infectious hematopoietic necrosis virus, Int. J. Biol. Macromol. 115 (2018) 1202-1210. https://doi.org/10.1016/j.ijbiomac.2018.04.132
Crossref Google Scholar
[24]

K. Mohan, S. Ravichandran, T. Muralisankar, et al., Potential uses of fungal polysaccharides as immunostimulants in fish and shrimp aquaculture: a review, Aquaculture 500 (2019) 250-263. https://doi.org/10.1016/j.aquaculture.2018.10.023
Crossref Google Scholar
[25]

F.G. Xia, J.H. Fan, Z. Mei, et al., Antioxidant effects of a water-soluble proteoglycan isolated from the fruiting bodies of Pleurotus ostreatus, J. Taiwan Inst. Chem. E. 42 (3) (2011) 402-407. https://doi.org/10.1016/j.jtice.2010.08.012
Crossref Google Scholar
[26]

C.W. Ma, M.Y. Feng, X.F. Zhai, et al., Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities, J. Taiwan Inst. Chem. E. 44 (6) (2013) 886-894. https://doi.org/10.1016/j.jtice.2013.01.032
Crossref Google Scholar
[27]

H.M. Qi, T.T. Zhao, Q.B. Zhang, et al., Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta), J. Appl. Phycol. 17 (2005) 527-534. https://doi.org/10.1007/s10811-005-9003-9
Crossref Google Scholar
[28]

H. Yang, J. Dai, S.W. Chen, et al., Study on isolation, purification, composition and immulogical characterics of Ganoderma lucidum body polysaccharides, Food and Machinery 31 (1) (2015) 132-136
Google Scholar
[29]

J. Shen, H.J. Wang, Y.M. Xia, Molecular simulation of model sulfated polysaccharides of low molecular weight from Ganoderma lucidum and their interaction with human serum albumin, Struct. Chem. 25 (2014) 1423-1435. https://doi.org/10.1007/s11224-014-0420-8
Crossref Google Scholar
Food Science and Human Wellness
Volume 10 Issue 4,
July 2021
Pages 508-513
DOI: 10.1016/j.fshw.2021.04.013
Cite this article:
Zhao S, Lei M, Xu H, et al. The normal cell proliferation and wound healing effect of polysaccharides from Ganoderma amboinense. Food Science and Human Wellness, 2021, 10(4): 508-513. https://doi.org/10.1016/j.fshw.2021.04.013

439

Views

40

Downloads

14

Crossref

15

Web of Science

17

Scopus

2

CSCD

Altmetrics
Received: 30 September 2020
Revised: 13 November 2020
Accepted: 23 November 2020
Published: 04 June 2021
© 2021 Beijing Academy of Food Sciences. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return