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Research Article | Open Access

A new Lactobacillus gasseri strain HMV18 inhibits the growth of pathogenic bacteria

Xiang Gaoa,b,1, Zixuan Wanga,1, Xiang Lic,1, Xiaoling Zhangd, Shengqiang Duc, Miaomiao Jiab,c, Dailun Hua, Xianxian Jiaa,b( ), Bin Congb,f( ), Yan Zhangb,e,f( ), Chunling Mab,f, Song Zhoud, Jun Zhangc
Department of Pathogen Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical Sciences, Shijiazhuang 050017, China
Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Shijiazhuang Great Wall Integrated Traditional Chinese and Western Medicine Hospital, Shijiazhuang 050035, China
Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China
College of Forensic Medicine, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medical University, Shijiazhuang 050017, China

1 These authors contributed equally to this work. Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

To search for a new eco-friendly therapy for infectious disease caused by Escherichia coli, Staphylococcus aureus or Klebsiella oxytoca, we collected the vaginal swabs from healthy women, screened for Lactobacillus and found a strain repressing the growth of pathogenic bacteria. The new isolate was identified as L. gasseri by the colony morphology, Gram staining, biochemical reactions and confirmed by the 16S rDNA sequencing. The HMV18 strain inhibited the growth of food-borne pathogens such as E. coli, S. aureus and K. oxytoca. The HMV18 strain was sensitive to penicillin, ampicillin, erythromycin, tetracycline and chloramphenicol. The HMV18 strain produced α-hemolysis. Pathological histology of the mice ileum showed that the mucosa, villi, lamina propria and crypt depth remained intact and there was no inflammation or hyperemia in the L. gasseri HMV18 gavaged group. L. gasseri HMV18 could not up-regulate inflammatory cytokines level of plasma. All the results suggested L. gasseri HMV18 is a candidate probiotic to be an additive for food preservation or drug to prevent food-borne diseases.

Keywords

BacteriaLactobacillus gasseriAntagonismSafety evaluation

References

[1]

S. Binda, C. Hill, E. Johansen, et al., Criteria to qualify microorganisms as "probiotic" in foods and dietary supplements, Front Microbiol. 11 (2020) 1662. https://doi.org/10.3389/fmicb.2020.01662.
Crossref Google Scholar
[2]

H. Hardy, J. Harris, E. Lyon, et al., Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immunopathology, Nutrients 5 (2013) 1869-1912. https://doi.org/10.3390/nu5061869.
Crossref Google Scholar
[3]

T. Hori, K. Matsuda, K. Oishi, Probiotics: a dietary factor to modulate the gut microbiome, host immune system, and gut-brain interaction, Microorganisms 8 (2020) 1401. https://doi.org/10.3390/microorganisms8091401.
Crossref Google Scholar
[4]

D. Ren, C. Li, Y. Qin, et al., Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests, Anaerobe 35 (2015) 22-27. https://doi.org/10.1016/j.anaerobe.2015.06.008.
Crossref Google Scholar
[5]

A. Feehan, J. Garcia-Diaz, Bacterial, gut microbiome-modifying therapies to defend against multidrug resistant organisms, Microorganisms 8 (2020) 166. https://doi.org/10.3390/microorganisms8020166.
Crossref Google Scholar
[6]

J. Hrdy, J. Alard, A. Couturier-Maillard, et al., Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses, Sci. Rep. 10 (2020) 5345. https://doi.org/10.1038/s41598-020- 62161-1.
Crossref Google Scholar
[7]

K. Hufnagl, I. Pali-Scholl, F. Roth-Walter, et al., Dysbiosis of the gut and lung microbiome has a role in asthma, Semin. Immunopathol. 42 (2020) 75-93. https://doi.org/10.1007/s00281-019-00775-y.
Crossref Google Scholar
[8]

A.H. Rad, L.A. Maleki, H.S. Kafil, et al., Postbiotics: a novel strategy in food allergy treatment, Crit. Rev. Food Sci. Nutr. 61 (2021) 492-499. https://doi.org/10.1080/10408398.2020.1738333.
Crossref Google Scholar
[9]

P. Markowiak-Kopec, K. Slizewska, The effect of probiotics on the production of short-chain fatty acids by human intestinal microbiome, Nutrients 12 (2020) 1107. https://doi.org/10.3390/nu12041107.
Crossref Google Scholar
[10]

H.J. Zheng, J. Guo, Q. Wang, et al., Probiotics, prebiotics, and synbiotics for the improvement of metabolic profiles in patients with chronic kidney disease: a systematic review and meta-analysis of randomized controlled trials, Crit. Rev. Food Sci. Nutr. 61 (2021) 577-598. https://doi.org/10.1080/10408398.2020.1740645.
Crossref Google Scholar
[11]

S.T. Talarico, F.E. Santos, K.G. Brandt, et al., Anaerobic bacteria in the intestinal microbiota of Brazilian children, Clinics (Sao Paulo) 72 (2017) 154-160. https://doi.org/10.6061/clinics/2017(03)05.
Crossref Google Scholar
[12]

M. Tanaka, J. Nakayama, Development of the gut microbiota in infancy and its impact on health in later life, Allergol. Int. 66 (2017) 515-522. https://doi.org/10.1016/j.alit.2017.07.010.
Crossref Google Scholar
[13]

M.J. Butel, A.J. Waligora-Dupriet, S. Wydau-Dematteis, The developing gut microbiota and its consequences for health, J. Dev. Orig. Health Dis. 9 (2018) 590-597. https://doi.org/10.1017/S2040174418000119.
Crossref Google Scholar
[14]

K. Selle, T.R. Klaenhammer, Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health, FEMS Microbiol. Rev. 37 (2013) 915-935. https://doi.org/10.1111/1574-6976.12021.
Crossref Google Scholar
[15]
M. Widmann, W. Zhang, Genomics of foodborne bacterial pathogens, Springer New York, (2011). https://doi.org/10.1007/978-1-4419-7686-4.
[16]

Y. Liu, H. Wu, Z.L. Sun, et al., Contamination and biofilm formation of foodborne and opportunistic pathogens in yellow-feathered chicken carcass, Foodborne Pathog. Dis. 18 (2021) 210-218. https://doi.org/10.1089/fpd.2020.2876.
Crossref Google Scholar
[17]

B. Hetzer, D. Orth-Holler, R. Wurzner, et al., Enhanced acquisition of antibiotic-resistant intestinal E. coli during the first year of life assessed in a prospective cohort study, Antimicrob. Resist. Infect. Control 8 (2019) 79. https://doi.org/10.1186/s13756-019-0522-6.
Crossref Google Scholar
[18]

A. Mazumdar, V. Adam, Antimicrobial peptides-an alternative candidates to antibiotics against Staphylococcus aureus and its antibiotic-resistant strains, J. Mol. Clin. Med. 4 (2021) 1-17. https://doi.org/10.31083/j.jmcm.2021.01.208.
Crossref Google Scholar
[19]

C. Lowe, B. Willey, A. O'Shaughnessy, et al., Outbreak of extendedspectrum β-lactamase–producing Klebsiella oxytoca infections associated with contaminated handwashing sinks, Emerg. Infect. Dis. 18 (2012) 1242-1247. https://doi.org/10.3201/eid1808.111268.
Crossref Google Scholar
[20]

P.G. Cano, G. Perdigon, Probiotics induce resistance to enteropathogens in a re-nourished mouse model, J. Dairy Res. 70 (2003) 433-440. https://doi.org/10.1017/s0022029903006472.
Crossref Google Scholar
[21]

T. Itoh, Y. Fujimoto, Y. Kawai, et al., Inhibition of food-borne pathogenic bacteria by bacteriocins from Lactobacillus gasseri, Lett. Appl. Microbiol. 21 (1995) 137-141. https://doi.org/10.1111/j.1472-765x.1995.tb01025.x.
Crossref Google Scholar
[22]

O.V. Rybalchenko, V.M. Bondarenko, O.G. Orlova, et al., Inhibitory effects of Lactobacillus fermentum on microbial growth and biofilm formation, Arch. Microbiol. 197 (2015) 1027-1032. https://doi.org/10.1007/s00203-015-1140-1.
Crossref Google Scholar
[23]

A.C. Anderson, M. Sanunu, C. Schneider, et al., Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing, BMC Microbiol. 14 (2014) 312. https://doi.org/10.1186/s12866-014-0312-5.
Crossref Google Scholar
[24]

W. Zhang, Z. Sun, Random local neighbor joining: a new method for reconstructing phylogenetic trees, Mol. Phylogenet. Evol. 47 (2008) 117-128. https://doi.org/10.1016/j.ympev.2008.01.019.
Crossref Google Scholar
[25]

S. Kumar, G. Stecher, K. Tamura, MEGA7: molecular evolutionary genetics analysis Version 7.0 for bigger datasets, Mol. Biol. Evol. 33 (2016) 1870-1874. https://doi.org/10.1093/molbev/msw054.
Crossref Google Scholar
[26]

S. Akhter, R.K. Aziz, R.A. Edwards, PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and compositionbased strategies, Nucleic Acids Res. 40 (2012) e126. https://doi.org/10.1093/nar/gks406.
Crossref Google Scholar
[27]

E. Yang, L. Fan, J. Yan, et al., Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria, AMB Express 8 (2018) 10. https://doi.org/10.1186/s13568-018-0536-0.
Crossref Google Scholar
[28]

E. McDevitt, F. Khan, A. Scasny, et al., Hydrogen peroxide production by Streptococcus pneumoniae results in alpha-hemolysis by oxidation of Oxyhemoglobin to Met-hemoglobin, mSphere 5 (2020). https://doi.org/10.1128/mSphere.01117-20.
Crossref Google Scholar
[29]

A. Reyes-Diaz, V. Mata-Haro, J. Hernandez, et al., Milk fermented by specific Lactobacillus strains regulates the serum levels of IL-6, TNF-alpha and IL-10 cytokines in a LPS-stimulated murine model, Nutrients 10 (2018) 691. https://doi.org/10.3390/nu10060691.
Crossref Google Scholar
Food Science and Human Wellness
Volume 11 Issue 2,
March 2022
Pages 247-254
DOI: 10.1016/j.fshw.2021.11.010
Cite this article:
Gao X, Wang Z, Li X, et al. A new Lactobacillus gasseri strain HMV18 inhibits the growth of pathogenic bacteria. Food Science and Human Wellness, 2022, 11(2): 247-254. https://doi.org/10.1016/j.fshw.2021.11.010

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Received: 19 October 2020
Revised: 19 December 2020
Accepted: 22 November 2021
Published: 25 November 2021
© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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