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Research Article | Open Access

Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography

Feiya Shenga,b,1, Songsong Wanga,1, Xiao Luoc, Jianbo Xiaoa, Linfeng Hud( ), Peng Lia( )
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, China
School of Basic Medical Sciences, Chengdu University, Chengdu 610106, China
Chengdu Institute for Food and Drug Control, Chengdu 610045, China
Chongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region, School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

1 These authors contributed equally to this work. Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma. In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.

Keywords

Quality assessmentGanodermaNucleosidesBasesMicellar electrokinetic chromatography

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Food Science and Human Wellness
Volume 11 Issue 2,
March 2022
Pages 263-268
DOI: 10.1016/j.fshw.2021.11.015
Cite this article:
Sheng F, Wang S, Luo X, et al. Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography. Food Science and Human Wellness, 2022, 11(2): 263-268. https://doi.org/10.1016/j.fshw.2021.11.015

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Received: 01 August 2020
Revised: 14 September 2020
Accepted: 21 September 2020
Published: 25 November 2021
© 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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