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Research Article | Open Access

Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells

Xiaojiao Jia, Jing Wangb, Aijin Mac, Duo Fenga, Yue Hea, Wenjie Yana( )
College of Biochemical Engineering, Beijing Union University, Beijing 100101, China
Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
College of Food and Health, Beijing Technology and Business University, Beijing 100048, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Objective

To investigate the effects of silkworm pupa (Bombyx mori) protein (SPP) on cell proliferation, apoptosis and energy metabolism in human colon cancer cells DLD-1.

Methods

CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group (normal cultured DLD-1 cells) and SPP dose groups; Annexin-V/PI was applied to observe cell apoptosis; XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function.

Results

Comparing with the control, SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested (P < 0.01); flow cytometry showed that SPP significantly promoted apoptosis (P < 0.05). Additionally, SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses.

Conclusion

SPP can cause oxidative damage, promote apoptosis, and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells, which reveals that SPP has the potential to serve as the anti-cancer drugs in the future, but further experimental evidence is needed.

Keywords

MitochondriaApoptosisGlycolysisColon cancer DLD-1 cellsSilkworm pupa protein

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Food Science and Human Wellness
Volume 11 Issue 5,
September 2022
Pages 1171-1176
DOI: 10.1016/j.fshw.2022.04.011
Cite this article:
Ji X, Wang J, Ma A, et al. Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells. Food Science and Human Wellness, 2022, 11(5): 1171-1176. https://doi.org/10.1016/j.fshw.2022.04.011

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Received: 11 December 2020
Revised: 21 December 2020
Accepted: 30 December 2020
Published: 02 June 2022
© 2022 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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