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Research Article | Open Access

Alteration of fecal microbiome and metabolome by mung bean coat improves diet-induced non-alcoholic fatty liver disease in mice

Dianzhi Houa,b, Jian Tanga, Meili Huanc, Fang Liud, Sumei Zhoua( ), Qun Shenb( )
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China
College of Food Science and Nutritional Engineering, Key Laboratory of Plant Protein and Grain processing, China Agricultural University, Beijing 100083, China
COFCO Nutrition and Health Research Institute, Beijing 102209, China
College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Dysbiosis of gut microbiota and its derived metabolites has been linked to the occurrence and development of nonalcoholic fatty liver disease. Our previous study has demonstrated that mung bean coat (MBC) might be mainly responsible for the beneficial effects of whole mung bean on high fat diet (HFD)-induced metabolic disorders. To investigate whether MBC, which is rich in dietary fiber and phytochemicals, can protect against HFD-induced hepatic steatosis in mice via targeting gut microbiota and its metabolites, we conducted this study. Results showed that MBC could effectively alleviative the obese phenotype, reduce the lipid accumulation and insulin resistance, and improve the hepatic oxidative stress and inflammatory response. Furthermore, MBC significantly prevented the HFD-induced changes in the structure and composition of gut microbiota, characterized by promoting the bloom of Akkermansia, Lachnospiraceae_NK4A136_group, and norank_f_Muribaculaceae, and along with the elevated short-chain fatty acids concentrations. Non-targeted metabolomic analysis indicated a metabolism disorder that was obviously improved by MBC via regulating sphingolipid metabolism and α-linolenic acid metabolism. These findings suggested that MBC could improve hepatic steatosis through manipulating the crosstalk between gut microbiota and its metabolites.

Keywords

Gut microbiotaShort-chain fatty acidsMetabolitesHepatic steatosisMBC

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Food Science and Human Wellness
Volume 11 Issue 5,
September 2022
Pages 1259-1272
DOI: 10.1016/j.fshw.2022.04.023
Cite this article:
Hou D, Tang J, Huan M, et al. Alteration of fecal microbiome and metabolome by mung bean coat improves diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022, 11(5): 1259-1272. https://doi.org/10.1016/j.fshw.2022.04.023

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Received: 17 November 2021
Revised: 20 December 2021
Accepted: 18 January 2022
Published: 02 June 2022
© 2022 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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