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Research Article | Open Access

Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis

Yue Yu, Fusheng Pei, Zhanming Li( )
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Plant flavonoids have received considerable attention for their health benefits. However, structure-activity relationships between flavonoids such as orientin and vitexin with similar structures are rarely reported. In the present study, molecular docking study suggested that orientin and vitexin suppressed inflammatory responses through the modulation of the mitogen-activated protein kinase (MAPK) signaling pathway. Moreover, RAW264.7 cells with lipopolysaccharide-induced inflammation were used to evaluate the anti-inflammatory activities of orientin and vitexin, based on the inflammation-related cytokines production, quantitative real-time reverse transcription PCR, and western blotting analysis. As a result, orientin or vitexin attenuated inflammatory responses through modulation of the MAPK/NF-κB signaling pathway by suppressing NF-κB translocation.

Keywords

Molecular dockingAnti-inflammatoryNF-κBPlant extractMitogen-activated protein kinase

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Food Science and Human Wellness
Volume 11 Issue 5,
September 2022
Pages 1273-1281
DOI: 10.1016/j.fshw.2022.04.024
Cite this article:
Yu Y, Pei F, Li Z. Orientin and vitexin attenuate lipopolysaccharide-induced inflammatory responses in RAW264.7 cells: a molecular docking study, biochemical characterization, and mechanism analysis. Food Science and Human Wellness, 2022, 11(5): 1273-1281. https://doi.org/10.1016/j.fshw.2022.04.024

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Received: 02 June 2020
Revised: 11 January 2021
Accepted: 05 February 2021
Published: 02 June 2022
© 2022 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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