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Research Article | Open Access

Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment

Dong Hea,b,1, Wen Zengb,1, Yi Wangb, Yifan Xingb, Kang Xiongb, Nan Sub, Chong Zhangb,c, Yuan Lub, Xinhui Xingb,c,d,e( )
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China
Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 440300, China

1 Authors contributed equally to the article.

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF) were found to be effective in UC prevention in dextran sulfate sodium-induced mice in vivo. Herein, 4 novel peptides are identified from AC-WGAF by membrane ultrafiltration, recycling preparative high-performance liquid chromatography, and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight mass spectrometry, possessing anti-colitis activity via using an in vitro model. One of those peptides named T24 (PVLGPVRGPFPLL) exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss, maintaining epithelial barrier integrity, and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling pathways. Thus, T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment.

Keywords

PeptideUlcerative colitisIntestinal epithelial barrierCell proliferationFermentation productMitogen-activated protein kinase signaling pathways

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Food Science and Human Wellness
Volume 11 Issue 6,
November 2022
Pages 1464-1474
DOI: 10.1016/j.fshw.2022.06.003
Cite this article:
He D, Zeng W, Wang Y, et al. Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment. Food Science and Human Wellness, 2022, 11(6): 1464-1474. https://doi.org/10.1016/j.fshw.2022.06.003

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Received: 19 August 2021
Revised: 22 September 2021
Accepted: 01 December 2021
Published: 18 July 2022
© 2022 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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