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Research Article | Open Access

Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags

Shen Yanga, Zijin Yuana, Jude Juventus Aweyab, Shanggui Dengc, Wuyin Wenga, Yueling Zhangb( ), Guangming Liua( )
College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Jimei University, Xiamen 361021, China
Department of Biology, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety. In this study, 5 species of bacteria, mainly gram-positive bacteria including three species of Bacillus spp., Arthrobacter arilaitensis and Staphylococcus warneri, were isolated and identified from bacteria-contaminated A. auricular culture bags. An in silico predicted antimicrobial peptide from the β-1,3-glucan-binding protein sequence of Penaeus vannamei, designated PvGBP2 (FLKLGRKSRYGMLKL), was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp. explored. The minimal inhibitory concentrations (MIC) of PvGBP2 on Bacillus spp. were 15.6–31.25 μg/mL. Peptide PvGBP2 could inhibit Bacillus subtilis in A. auricular culture bags to maintain growth and yield of A. auricular. Transmission electron microscopy (TEM) revealed that PvGBP2 kills bacteria by perforating the cell wall, destroying membrane integrity and resulting in the leakage of intracellular solutes. In addition, PvGBP2 inhibits biofilm formation by B. subtilis by 90.6% at 1 × MIC. Thus, peptide PvGBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A. auricular cultivation and the spread of foodborne pathogens.

Keywords

Antimicrobial peptideAuricularia auricularPenaeus vannameiβ-1,3-Glucan-binding proteinBacillus spp.e

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Food Science and Human Wellness
Volume 11 Issue 6,
November 2022
Pages 1607-1613
DOI: 10.1016/j.fshw.2022.06.019
Cite this article:
Yang S, Yuan Z, Aweya JJ, et al. Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags. Food Science and Human Wellness, 2022, 11(6): 1607-1613. https://doi.org/10.1016/j.fshw.2022.06.019

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Received: 15 April 2021
Revised: 16 May 2021
Accepted: 08 July 2021
Published: 18 July 2022
© 2022 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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