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Research Article | Open Access

Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis

Peilin Chena,b, Lu Liub, Zirun Chengb, Yi Zhanga,b,c, Baodong Zhenga,b,c, Xiaoke Hud( ), Hongliang Zenga,b,c( )
Engineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction (PHP2) isolated from the red marine alga Porphyra haitanensis. PHP2 was characterized as a sulfated glucogalactan, with a hypothetical backbone structure of →4)Gα(1→6)G4Sβ(1→4)Glc(1→ and a side chain of Man(1→6)Glc. PHP2 had an irregular spherical chain conformation. The 16S rRNA sequence analysis revealed that PHP2 modulated the rat fecal micro-flora composition, with a similar effect to inulin, changing the dominant genus (Lactobacillus and Escherichia-Shigella) and promoting the growth of organisms that degrade sulfur-containing polysaccharides, such as Desulfovibrio, Ruminococcaceae_UCG-005, and Ruminococcus_2. PHP2 can promote production of acetic, propionic and butyric acid by rat fecal micro-flora. Prediction of metabolic function suggested that PHP2 could modulate cholesterol metabolism. The sulfated glucogalactan fermentation behavior may be associated with its monosaccharide composition, chain branching and chain conformation. PHP2 appeared to have considerable potential as functional food, and was associated with sulfur-containing polysaccharides in general.

Keywords

Structure characterizationPolysaccharideShort-chain fatty acidsPorphyra haitanensisFecal microfloraMetabolic function prediction

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Food Science and Human Wellness
Volume 12 Issue 2,
March 2023
Pages 596-606
DOI: 10.1016/j.fshw.2022.07.062
Cite this article:
Chen P, Liu L, Cheng Z, et al. Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis. Food Science and Human Wellness, 2023, 12(2): 596-606. https://doi.org/10.1016/j.fshw.2022.07.062

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Received: 29 April 2021
Revised: 12 June 2021
Accepted: 10 August 2021
Published: 07 September 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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