AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Food Science and Human Wellness Article
PDF (1.3 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice

Junjuan Wanga, Cui Zhoub, Shiwen Hana, Zainabu Majida, Na Sunc, Huilian Chea( )
Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
School of Public Health, Capital Medical University, Beijing 100069, China
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China

Peer review under responsibility of KeAi Communications Co., Ltd.

Show Author Information

Abstract

The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions (Ca2+) in peanut allergy remains unknown. C3H/HeJ mice sensitized with peanut protein extract (PPE) were injected with anti-CD4 antibodies for 4 weeks. Stimulation with PPE increased the specific immunoglobulin (IgE), cytokine, histamine, and mMcp-1 levels, upregulated decorin (Dcn) expression, induced Ca2+ inflow in the spleen, and augmented the expression of the transcription factors GATA-3 and Foxp3, which resulted in Th2 and Treg cell activation. Notably, the Ca2+ levels were positively correlated with the histamine, interleukin (IL)-4, IL-5, and IL-13 levels, and negatively correlated with IL-10 levels. However, administration of anti-CD4 antibodies markedly alleviated allergic symptoms, activated T cells, and reduced Ca2+ inflow, cytokine, histamine, mMcp-1, and the IgHG3, CXCL12, MMP2 and FABP4 gene. Our results indicated that anti-CD4 antibodies can ameliorate PPE-induced allergy, which is probably related to the suppression of Ca2+ inflow, and inhibiting histamine, cytokine and IgHG3, CXCL12, MMP2, and FABP4, thus exerting a protective effect against PPE-sensitized food allergy.

Keywords

AllergyCalcium ionsAnti-CD4C3H/HeJ micePeanut

References

[1]

T.B. Thottingal, B.P. Stefura, F.E. Simons, et al., Human subjects without peanut allergy demonstrate T cell-dependent, TH2-biased, peanut-specific cytokine and chemokine responses independent of TH1 expression, J. Allergy Clin. Immunol. 118 (2006) 905-914. https://doi.org/10.1016/j.jaci.2006.06.016.
Crossref Google Scholar
[2]

J. Wang, A.H. Liu, Food allergies and asthma, Curr. Opin. Allergy Clin. Immunol. 11 (2011) 249-254. https://doi.org/10.1097/ACI.0b013e3283464c8e.
Crossref Google Scholar
[3]

T. Zhang, Y. Shi, Y. Zhao, et al., Boiling and roasting treatment affecting the peanut allergenicity, Ann. Transl. Med. 6 (2018) 357. https://doi.org/10.21037/atm.2018.05.08.
Crossref Google Scholar
[4]

V. Robert, E. Triffaux, M. Savignac, et al., Calcium signalling in T-lymphocytes, Biochimie 93 (2011) 2087-2094. https://doi.org/10.1016/j.biochi.2011.06.016.
Crossref Google Scholar
[5]

S. Feske. Calcium signalling in lymphocyte activation and disease, Nat. Rev. Immunol. 7 (2007) 690-702. https://doi.org/10.1038/nri2152.
Crossref Google Scholar
[6]

F. Shah, A. Shi, J. Ashley, et al., Peanut allergy: characteristics and approaches for mitigation, Compr. Rev. Food. Sci. F. 18 (2019) 1361-1387. https://doi.org/10.1111/1541-4337.12472.
Crossref Google Scholar
[7]

C.H. Wolff, S.C. Hong, H.von Grafenstein, et al., TCR-CD4 and TCR-TCR interactions as distinctive mechanisms for the induction of increased intracellular calcium in T-cell signalling, J. Immunol. 151 (1993) 1337-1345.
Crossref Google Scholar
[8]

P.L. Yarova, A.L. Stewart, V. Sathish, et al., Calcium-sensing receptor antagonists abrogate airway hyperresponsiveness and inflammation in allergic asthma, Sci. Transl. Med. 7 (2015) 284ra260. https://doi.org/10.1126/scitranslmed.aaa0282.
Crossref Google Scholar
[9]

K.Y. Chiu, J.G. Li, Y. Lin., Calcium channel blockers for lung function improvement in asthma: a systematic review and meta-analysis, Ann. Allergy Asthma Immunol. 119 (2017) 518-523.e513. https://doi.org/10.1016/j.anai.2017.08.013.
Crossref Google Scholar
[10]

M.D. Cabral, P.E. Paulet, V. Robert, et al., Knocking down Cav1 calcium channels implicated in Th2 cell activation prevents experimental asthma, Am. J. Respir. Crit. Care. Med. 181 (2010) 1310-1317. https://doi.org/10.1164/rccm.200907-1166OC.
Crossref Google Scholar
[11]

B. Gomes, M.D. Cabral, A. Gallard, et al., Calcium channel blocker prevents T helper type 2 cell-mediated airway inflammation, Am. J. Respir. Crit. Care. Med. 175 (2007) 1117-1124. https://doi.org/10.1164/rccm.200607-1026OC.
Crossref Google Scholar
[12]

S.X. Qin, M. Wise, S.P. Cobbold, et al., Induction of tolerance in peripheral T cells with monoclonal antibodies, Eur. J. Immunol. 20 (1990) 2737-2745. https://doi.org/10.1002/eji.1830201231.
Crossref Google Scholar
[13]

E. Liu, H. Moriyama, J. Paronen, et al., Nondepleting anti-CD4 monoclonal antibody prevents diabetes and blocks induction of insulin autoantibodies following insulin peptide B:9-23 immunization in the NOD mouse, J. Autoimmun. 21 (2003) 213-219. https://doi.org/10.1016/s0896-8411(03)00116-1.
Crossref Google Scholar
[14]

A. Agua-Doce, L. Graca, Prevention of house dust mite induced allergic airways disease in mice through immune tolerance, PLoS ONE 6 (2011) e22320. https://doi.org/10.1371/journal.pone.0022320.
Crossref Google Scholar
[15]

L. Li, M. Crowley, A. Nguyen, et al., Ability of a nondepleting anti-CD4 antibody to inhibit Th2 responses and allergic lung inflammation is independent of coreceptor function, J. Immunol. 163 (1999) 6557-6566.
Crossref Google Scholar
[16]

S. Geng, Y.D. Gao, J. Yang, et al., Potential role of store-operated Ca2+ entry in Th2 response induced by histamine in human monocyte-derived dendritic cells, Int. Immunopharmacol. 12 (2012) 358-367. https://doi.org/10.1016/j.intimp.2011.12.008.
Crossref Google Scholar
[17]

J. Duarte, M. Caridade, L. Graca., CD4-blockade can induce protection from peanut-induced anaphylaxis, Front Immunol. 2 (2011) 56. https://doi.org/10.3389/fimmu.2011.00056.
Crossref Google Scholar
[18]

P. Lianto, S. Han, X. Li, et al., Quail egg homogenate alleviates food allergy induced eosinophilic esophagitis like disease through modulating PAR-2 transduction pathway in peanut sensitized mice, Sci. Rep. 8 (2018) 1049. https://doi.org/10.1038/s41598-018-19309-x.
Crossref Google Scholar
[19]

S.A. Bakheet, M.Z. Alzahrani, M.A. Ansari, et al., Resveratrol ameliorates dysregulation of Th1, Th2, Th17, and T regulatory cell-related transcription factor signaling in a BTBR T + tf/J mouse model of autism, Mol. Neurobiol. 54 (2017) 5201-5212. https://doi.org/10.1007/s12035-016-0066-1.
Crossref Google Scholar
[20]

K. Nishida, S. Yamasaki, Y. Ito, et al., FcɛRI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane, J. Cell Biol. 170 (2005) 115-126. https://doi.org/10.1083/jcb.200501111.
Crossref Google Scholar
[21]

S.N. Christo, K.R. Diener, R.E. Nordon, et al., Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus, Sci. Rep. 5 (2015) 7760. https://doi.org/10.1038/srep07760.
Crossref Google Scholar
[22]

K. Jeong, S.Y. Lee, K. Ahn, et al., A multicenter study on anaphylaxis caused by peanut, tree nuts, and seeds in children and adolescents, Allergy 72 (2017) 507-510. https://doi.org/10.1111/all.13096.
Crossref Google Scholar
[23]

J.O. Hourihane, K.J. Allen, W.G. Shreffler, et al., Peanut allergen threshold study (PATS): novel single-dose oral food challenge study to validate eliciting doses in children with peanut allergy, J. Allergy Clin. Immunol. 139 (2017) 1583-1590. https://doi.org/10.1016/j.jaci.2017.01.030.
Crossref Google Scholar
[24]

A.B. Simpson, E. Yousef, J. Hossain. Association between peanut allergy and asthma morbidity, J. Pediatr. 156 (2010) 777-781. https://doi.org/10.1016/j.jpeds.2009.11.080.
Crossref Google Scholar
[25]

P. Hemon, Y. Renaudineau, M. Debant, et al., Calcium signaling: from normal B cell development to tolerance breakdown and autoimmunity, Clin. Rev. Allergy Immunol. 53 (2017) 141-165. https://doi.org/10.1007/s12016-017-8607-6.
Crossref Google Scholar
[26]

R. Houtman, R. Ten Broeke, J.E. Blalock, et al., Attenuation of very late antigen-5-mediated adhesion of bone marrow-derived mast cells to fibronectin by peptides with inverted hydropathy to EF-hands, J. Immunol. 166 (2001) 861-867. https://doi.org/10.4049/jimmunol.166.2.861.
Crossref Google Scholar
[27]

C. Yang, X. Mo, J. Lv, et al., Lipopolysaccharide enhances FcɛRI-mediated mast cell degranulation by increasing Ca2+ entry through store-operated Ca2+ channels: implications for lipopolysaccharide exacerbating allergic asthma, Experimental Physiology 97 (2012) 1315-1327.
Crossref Google Scholar
[28]

H. Qiao, M.V. Andrade, F.A. Lisboa, et al., FcϵR1 and toll-like receptors mediate synergistic signals to markedly augment production of inflammatory cytokines in murine mast cells, Blood 107 (2006) 610-618. https://doi.org/10.1016/j.jaci.2005.12.1253.
Crossref Google Scholar
[29]

W.C. Chang, C. Nelson, A.B. Parekh, Ca2+ influx through CRAC channels activates cytosolic phospholipase A2, leukotriene C4 secretion, and expression of c-fos through ERK-dependent and -independent pathways in mast cells, Faseb J. 20 (2006) 2381-2383. https://doi.org/10.1096/fj.06-6016fje.
Crossref Google Scholar
[30]

N. Suganuma, S. Ito, H. Aso, et al., STIM1 regulates platelet-derived growth factor-induced migration and Ca2+ influx in human airway smooth muscle cells. PLoS ONE, 7 (2012) e45056. https://doi.org/10.1371/journal.pone.0045056.
Crossref Google Scholar
[31]

J.C. Kashiwakura, T. Ando, K. Matsumoto, et al., Histamine-releasing factor has a proinflammatory role in mouse models of asthma and allergy, J. Clin. Invest. 122 (2012) 218-228. https://doi.org/10.1172/jci59072.
Crossref Google Scholar
[32]

Y.C. Lee, K.H. Lee, H.B. Lee, et al., Serum levels of interleukins (IL)-4, IL-5, IL-13, and interferon-gamma in acute asthma, J. Asthma 38 (2001) 665-671. https://doi.org/10.1081/jas-100107544.
Crossref Google Scholar
[33]

P. Wang, W. Zhao, J. Sun, et al., Inflammatory mediators mediate airway smooth muscle contraction through a G protein-coupled receptor-transmembrane protein 16A-voltage-dependent Ca2+ channel axis and contribute to bronchial hyperresponsiveness in asthma, J. Allergy Clin. Immunol. 141 (2018) 1259-1268.e1211. https://doi.org/10.1016/j.jaci.2017.05.053.
Crossref Google Scholar
[34]

M.C. Borges, V. Narayanan, R.V. Iozzo, et al., Deficiency of decorin induces expression of Foxp3 in CD4+ CD25+ T cells in a murine model of allergic asthma, Respirology 20 (2015) 904-911. https://doi.org/10.1111/resp.12485.
Crossref Google Scholar
[35]

M. Oh-hora, A. Rao, Calcium signaling in lymphocytes. Curr. Opin. Immunol. 20 (2008) 250-258. https://doi.org/10.1016/j.coi.2008.04.004.
Crossref Google Scholar
[36]

X.C. Liao, D.R. Littman, Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk, Immunity 3 (1995) 757-769. https://doi.org/10.1016/1074-7613(95)90065-9.
Crossref Google Scholar
[37]

W. Zhang, C.L. Sommers, D.N. Burshtyn, et al., Essential role of LAT in T cell development, Immunity 10 (1999) 323-332. https://doi.org/10.1016/s1074-7613(00)80032-1.
Crossref Google Scholar
[38]

H.J. Shin, J.B. Lee, S.H. Park, et al., T-bet expression is regulated by EGR1-mediated signaling in activated T cells, Clin. Immunol. 131 (2009) 385-394. https://doi.org/10.1016/j.clim.2009.02.009.
Crossref Google Scholar
[39]
N.R. Deshpande, Impact of time on the size, shape and effector responses of Cd4+ T cells. University of Arizona, (2015).
[40]

C.T. Mayer, L. Tian, C. Hesse, et al. Anti-CD4 treatment inhibits autoimmunity in scurfy mice through the attenuation of co-stimulatory signals, J. Autoimmun 50 (2014) 23-32. https://doi.org/10.1016/j.jaut.2013.08.010.
Crossref Google Scholar
[41]

C.P. Frossard, L. Steidler, P.A. Eigenmann, Oral administration of an IL-10-secreting Lactococcus lactis strain prevents food-induced IgE sensitization, J. Allergy Clin. Immunol. 119 (2007) 952-959. https://doi.org/10.1016/j.jaci.2006.12.615.
Crossref Google Scholar
[42]

A.B. Sanchez, K.E. Medders, R. Maung, et al., CXCL12-induced neurotoxicity critically depends on NMDA receptor-gated and L-type Ca2+ channels upstream of p38 MAPK, J. Neuroinflamm. 13 (2016) 252. https://doi.org/10.1186/s12974-016-0724-2.
Crossref Google Scholar
[43]

J. Girona, R. Rosales, N. Plana, et al., FABP4 induces vascular smooth muscle cell proliferation and migration through a MAPK-dependent pathway, PLoS ONE, 8 (2013) e81914. https://doi.org/10.1371/journal.pone.0081914.
Crossref Google Scholar
Food Science and Human Wellness
Volume 12 Issue 3,
May 2023
Pages 765-773
DOI: 10.1016/j.fshw.2022.09.010
Cite this article:
Wang J, Zhou C, Han S, et al. Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice. Food Science and Human Wellness, 2023, 12(3): 765-773. https://doi.org/10.1016/j.fshw.2022.09.010

582

Views

40

Downloads

3

Crossref

2

Web of Science

0

Scopus

0

CSCD

Altmetrics
Received: 03 July 2020
Revised: 29 July 2020
Accepted: 05 October 2020
Published: 15 October 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return