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Research Article | Open Access

Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro

Zihao Xua,b, Hao Baia,b, Xin Maa,b, Yong Wua,c,d, Zhihua Wua,c,d, Anshu Yanga,c,d, Weixiang Maoe( ), Xin Lia,b,d( ), Hongbing Chena,c,d
State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China
School of food science and Technology, Nanchang University, Nanchang 330047, China
Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China
Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China
Jiangxi Institute of Quality and Standardization, Nanchang 330046, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Milk allergy is a common allergic reaction found in infants and young children, most of them appear tolerance after growing up. In this study, infant formula was digested by simulated in vitro digestion method. The potential non-allergenic peptides were further screened from undigested products by exclusion of the known epitopes from β-lactoglobulin (BLG) and α-lactalbumin (ALA). These potential non-allergenic peptides were synthesized and their transferability were determined by Caco-2 cell monolayer model. Finally, the potential allergenicity were evaluated by KU812 cell degranulation model. The results showed that 7 peptides were screened as non-allergenic sequences, among which were 3 from ALA and 4 from BLG. The Caco-2 cell model showed that all the synthetic peptides were absorbed and transported well. However, only peptide BLG107-118 showed potential allegencity by KU812 model. In conclusion, six peptides, including ALA29–51, ALA80–90, ALA94–103, BLG1–20, BLG24–50, and BLG123–139 were evaluated as hypoallergenic peptides, which could be used for candidates of peptides inducing immune tolerance for persons with milk allergy.

Keywords

Immune tolerancePeptidesMilk allergyEpitopesHypoallergenic

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Food Science and Human Wellness
Volume 12 Issue 3,
May 2023
Pages 817-824
DOI: 10.1016/j.fshw.2022.09.020
Cite this article:
Xu Z, Bai H, Ma X, et al. Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro. Food Science and Human Wellness, 2023, 12(3): 817-824. https://doi.org/10.1016/j.fshw.2022.09.020

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Received: 21 May 2021
Revised: 12 July 2021
Accepted: 21 December 2021
Published: 15 October 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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