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Research Article | Open Access

Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+

Qiao Baia,1, Xiaoping Fenga,1, Yu Wanga, Chunyu Wua, Ye Liub, Jiao Sunc, Tianli Yuea( ), Fangyu Longa( )
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
Beijing Key Laboratory of Plant Resource Research and Development, Beijing Technology and Business University, Beijing 100048, China
Department of Breast Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, China

1 Both authors contributed equally.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

Jujube contains abundant cyclic adenosine monophosphate (cAMP) and the ultrasonic-assisted pectinase extraction (UAPE) conditions for obtaining the maximum cAMP yield from jujube were optimized. Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield. The results showed that the optimal cAMP yield (783.0 μg/g) was derived at ratio of liquid to solid 5 mL/g, ratio of pectinase to raw material 1.5%, time 60 min and temperature 40 ℃. Moreover, the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E (IgE) and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia (RBL-2H3) cells using dinitrophenylated (DNP)-bovine serum albumin (BSA)-IgE. The results showed that cAMP interfered with sensitized cells, effectively inhibited the occurrence of basophil degranulation in dose dependence, and significantly reduced the activity of β-hexosamindase (β-hex), at the optimal concentration of 50 μg/mL. The level of anti-inflammatory factor interleukin-10 (IL-10) was promoted and the content of pro-inflammatory factor tumor necrosis factor-α (TNF-α) was suppressed by cAMP. In addition, influx of intracellular Ca2+ was repressed effectively. Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca2+, with the prevention of allergy symptoms.

Keywords

AllergyUltrasonic-assisted pectinase extractionCyclic adenosine monophosphateRat basophilic leukemia cellsCa2+ influx

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Food Science and Human Wellness
Volume 12 Issue 3,
May 2023
Pages 832-841
DOI: 10.1016/j.fshw.2022.09.023
Cite this article:
Bai Q, Feng X, Wang Y, et al. Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca2+. Food Science and Human Wellness, 2023, 12(3): 832-841. https://doi.org/10.1016/j.fshw.2022.09.023

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Received: 26 May 2021
Revised: 03 August 2021
Accepted: 11 October 2021
Published: 15 October 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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