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Research Article | Open Access

Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach

Mohan Lia,1, Qilong Lib,1, Haikun Yua, Xiumin Zhangc, Dehao Lid, Wanying Songa, Yan Zhenga( ), Xiqing Yuea( )
College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China
Beijing Academy of Food Sciences, Beijing 100068, China
The Fourth Affiliated Hospital of China Medical University, Shenyang 110866, China

1 These authors contributed equally to this work and should be regarded as co-first author.Peer review under responsibility of KeAi Communications Co., Ltd.]]>

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Abstract

This study aimed to analyze and compare the differentially expressed whey proteins (DEWPs) of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics. A total of 620 and 696 whey proteins were characterized in the donkey and bovine colostrum, respectively, including 383 common whey proteins. Among these common proteins, 80 were identified as DEWPs, including 21 upregulated and 59 downregulated DEWPs in donkey colostrum compared to bovine colostrum. Gene Ontology analysis revealed that these DEWPs were mainly related to cellular components, such as extracellular exosome, plasma membrane, and mitochondrion; biological processes, such as oxidation-reduction process, cell-cell adhesion, and small guanosine triphosphate (GTP) ase-mediated signal transduction; and molecular functions, such as GTP binding, GTPase activity, and soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor activity. Metabolic pathway analysis suggested that the majority of the DEWPs were associated with soluble NSF factor attachment protein receptor interactions in vesicular transport, fatty acid biosynthesis, and estrogen signaling pathways. Our results provide a vital insight into the differences between donkey and bovine colostrum, along with important information on the significant components as nutritional and functional factors to be included in infant formula based on multiple milk sources.

Keywords

ProteomicsBovine colostrumDonkey colostrumWhey proteinGene OntologyKyoto Encyclopedia of Genes and Genomes

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1224-1231
DOI: 10.1016/j.fshw.2022.10.004
Cite this article:
Li M, Li Q, Yu H, et al. Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach. Food Science and Human Wellness, 2023, 12(4): 1224-1231. https://doi.org/10.1016/j.fshw.2022.10.004

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Received: 03 January 2021
Revised: 09 February 2021
Accepted: 07 March 2021
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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