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Research Article | Open Access

Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

Junmei Maa, Sufang Fana, Liqiong Yanga, Liangna Hea, Hongwen Zhaia, Xiaowei Rena, Qiang Lia( ), Yan Zhanga,b( )
Hebei Food Inspection and Research Institute, Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Shijiazhuang 050200, China
Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) were developed. The samples were extracted with acetonitrile/acetic acid (99:1, V/V), and clean-up by SinChERS-Nano (single-step, cheap, effective, rugged, safe, nano) column, determined by UPLC-Q-TOF/MS. The accurate mass database and MS/MS database which contains 420 pesticides were established, the automatic retrieval of detection results was carried on according to the accurate mass, retention time, isotope ratio, ion fragment information, and so on. Method verification was performed on leeks samples. The results showed that 420 pesticides had good linearity in the range of 0.1–100 μg/L, and the correlation coeffificients (R2) was greater than 0.990. The limits of detections (LODs) and limits of quantifications (LOQs) of 420 pesticides were in range of 0.05–2.0 and 0.1–5.0 μg/L, respectively. The average spike recoveries at 3 levels were 70.1% to 119.7%, and the relative standard deviations (RSD) were lower than 20% (n = 6). With this method, a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables, which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public. This method was simple, sensitive and accurate, and could be used for rapid screening of 420 pesticide residues in fruits and vegetables.

Keywords

UPLC-Q-TOF/MSSinChERS-Nano columnPesticide residuesFruits and vegetables

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1064-1070
DOI: 10.1016/j.fshw.2022.10.024
Cite this article:
Ma J, Fan S, Yang L, et al. Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry. Food Science and Human Wellness, 2023, 12(4): 1064-1070. https://doi.org/10.1016/j.fshw.2022.10.024

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Received: 16 December 2021
Revised: 27 February 2022
Accepted: 18 March 2022
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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