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Research Article | Open Access

Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro

Xiaoyu Caia, Songxue Wanga, Huali Wangb, Suwen Liuc, Guishan Liud, Huibin Chene, Ji Kanga( ), Hao Wanga( )
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
China National Center for Food Safety Risk Assessment, Beijing 100022, China
College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
School of Food and Wine, Ningxia University, Yinchuan 750021, China
College of Life Sciences, Fujian Normal University, Fuzhou 350117, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells. In this study, SD rats were divided into the normal chow diet group (NCD), high fat diet group (HFD), three treatment groups feeding high-fat diet with naringenin (100, 200, 400 mg/kg) for 12 weeks. Results indicated that naringenin treatment decreased total cholesterol (TC), triglyceride (TG) and the non-high-density lipoprotein cholesterol (non-HDL-C) levels in serum. Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats. In addition, naringenin (25−75 µg/mL) decrease TG and TC levels in 3T3 mature adipocytes. The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology. Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes. Meanwhile, naringenin increased the AMPK (AMP-activated protein kinase) activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes. And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C. Molecular docking results indicated that naringenin could bind to AMPK protein. These results indicated naringenin reduced lipid accumulation through AMPK pathway.

Keywords

NaringeninLipid accumulationMolecular docking

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Food Science and Human Wellness
Volume 12 Issue 4,
July 2023
Pages 1174-1183
DOI: 10.1016/j.fshw.2022.10.043
Cite this article:
Cai X, Wang S, Wang H, et al. Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro. Food Science and Human Wellness, 2023, 12(4): 1174-1183. https://doi.org/10.1016/j.fshw.2022.10.043

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Received: 10 October 2021
Revised: 18 November 2021
Accepted: 08 January 2022
Published: 18 November 2022
© 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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