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Research Article | Open Access

Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis

Ruyu Shia,b, Xing Lia, Bing Gaoa, Chunhong Zhanga,c( ), Minhui Lia,b,c( )
Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China
Pharmaceutical Laboratory, Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, China
Inner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs, Baotou Medical College, Baotou 014040, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetes-related conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis.

Keywords

Diabetes mellitusCymbaria daurica L.Phenylethanoid glycosidesAlloxan-induced cell injury

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Food Science and Human Wellness
Volume 12 Issue 5,
September 2023
Pages 1580-1589
DOI: 10.1016/j.fshw.2023.02.016
Cite this article:
Shi R, Li X, Gao B, et al. Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis. Food Science and Human Wellness, 2023, 12(5): 1580-1589. https://doi.org/10.1016/j.fshw.2023.02.016

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Received: 24 May 2021
Revised: 04 June 2021
Accepted: 29 June 2021
Published: 21 March 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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