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Research Article | Open Access

Site-specific N-glycosylation characterization and allergenicity analysis of globulin-1 S allele from wheat

Linglin Fua, Rongrong Wanga, Jinru Zhoua,b, Chong Wanga, Yanbo Wanga()
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
Zhejiang Engineering Research Institute of Food & Drug Quality and Safety, School of Management and E-Business, Zhejiang Gongshang University, Hangzhou 310018, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

N-glycans in many proteins are of great concern because of their strong association with food allergies. Triticum aestivum (bread wheat), a major food crop, is known as one of the "Big Eight" allergenic groups. However, little research has been done about N-glycans in wheat glycoproteins. In this study, a soluble wheat glycoprotein was purified from wheat and further identified as globulin-1 S allele (GSA). The wheat GSA displayed significant IgE-binding activity. Moreover, one N-glycosylation site and 6 kinds of N-glycans were identified by mass spectrometry, including 3 high mannose types and 3 complex types. Furthermore, the IgE-binding activity of wheat GSA is proved to be reduced by the removal of N-glycan, thermal treatment (temperatures > 80 ℃), and strong acidic treatment (pH 3.0). These findings would provide a better understanding of the effects of N-glycosylation, thermal treatment, and acidic treatment on the molecular characteristics of GSA, and further provide new insights into the development of hypoallergenic wheat products.

Keywords

Triticum aestivumAllergenGlycoproteinN-glycansN-glycosylationC18-RPLC-MS/MS

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Food Science and Human Wellness
Volume 12 Issue 5,
September 2023
Pages 1601-1608
DOI: 10.1016/j.fshw.2023.02.020
Cite this article:
Fu L, Wang R, Zhou J, et al. Site-specific N-glycosylation characterization and allergenicity analysis of globulin-1 S allele from wheat. Food Science and Human Wellness, 2023, 12(5): 1601-1608. https://doi.org/10.1016/j.fshw.2023.02.020
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Structures and compositions of N-glycans identified in GSA.

No.Theoretical mass (Da)Observed mass (Da)CompositionProposed structure
Note: Hex represents hexose; HexNAc represents n-acetyl glucosamine; the blue square represents GlcNAc; the green circle represents Man; the yellow circle represents galactose; the red triangle represents fucose.
11223.0607611223.059448Hex3HexNAc2Galactose
21385.1136041385.112915Hex6HexNAc2
31466.1400251466.137329Hex7HexNAc2
41142.0343371142.032959Hex3HexNAc2
5980.774859980.778198Hex4HexNAc3Fucose2
61304.0871831304.086914Hex4HexNAc2Galactose