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Research Article | Open Access

Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection

Jiale Gaoa,1, Xiaodong Sunb,1, Yongxin Liua, Bing Niua, Qin Chena( ), Xueen Fangc( )
Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China
Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai 200444, China
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China

1 Contribute equally to this work.

Peer review under responsibility of KeAi Communications Co., Ltd.

Show Author Information

Abstract

The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework (MOF) was synthesized and applied in loop-mediated isothermal amplification (named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity (5 ng/μL for peanuts and 10 ng/μL for soybeans) and reliable repeatability (The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on.

Keywords

Loop-mediated isothermal amplificationCu-TCPP nanosheetPlant allergenNucleic acid detection

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Food Science and Human Wellness
Volume 12 Issue 5,
September 2023
Pages 1788-1798
DOI: 10.1016/j.fshw.2023.02.031
Cite this article:
Gao J, Sun X, Liu Y, et al. Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection. Food Science and Human Wellness, 2023, 12(5): 1788-1798. https://doi.org/10.1016/j.fshw.2023.02.031

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Received: 29 November 2021
Revised: 27 December 2021
Accepted: 13 May 2021
Published: 21 March 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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