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Research Article | Open Access

Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport

Mengmeng Wanga, Min Yua,b, Amel Thanina Amrouchea, Fan Jiea, Shengyang Jia, Baiyi Lua()
National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

7-Ketophytosterols are the major oxidation products of phytosterols in foods, which have been associated with atherosclerosis. However, their absorption mechanism remains unclear. The aim of our work was to investigate the absorption mechanism of 7-ketophytosterols and their effects on the cholesterol transport using Caco-2 cell model. The absorption percentage of 7-ketositosterol and 7-ketocampesterol was 1.16 %−1.68 % and 1.18 %−2.23 % respectively in the Caco-2 model, which is higher than that of their parent phytosterols, but lower than cholesterol-d7. The apparent permeability of 7-ketositosterol and 7-ketocampesterol at 30 μmol/L in the basolateral (BL)-to-apical (AP) direction were 0.42- and 0.55-fold of that in the AP-to-BL direction, indicating an active intake in the permeation mechanism of 7-ketophytosterols. Ezetimibe could significantly inhibit the transport of 7-ketophytosterols (P < 0.05), which means that their transport depends on niemann-pick c1-like 1 (NPC1L1) protein. The transport of cholesterol-d7 was significantly inhibited by 7-ketophytosterols (P < 0.05). Taken together, this study deepened our understanding of the absorption mechanism of common food-born 7-ketophytosterols and provides useful information on the inhibition of 7-ketophytosterols absorption.

Keywords

7-KetophytosterolsAbsorption mechanismCholesterol absorptionNiemann-pick c1-like 1

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Food Science and Human Wellness
Volume 12 Issue 5,
September 2023
Pages 1701-1707
DOI: 10.1016/j.fshw.2023.02.032
Cite this article:
Wang M, Yu M, Amrouche AT, et al. Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport. Food Science and Human Wellness, 2023, 12(5): 1701-1707. https://doi.org/10.1016/j.fshw.2023.02.032
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