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Research Article | Open Access

Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food

Pengfei Chena,1, Chaoqiong Hua,1, Xuan Taoa,b, Zheng Zhoua, Lijun Wanga,c, Xiao Yanga,c, Zhenming Chea, Xianggui Chena,c( ), Yukun Huanga,c( )
Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, School of Food and Biological Engineering, Xihua University, Chengdu 610039, China
Chengdu Brilliant Pharmaceutical Co., Ltd., Chengdu 610041, China
Key Laboratory of Food Non-Thermal Processing, Engineering Technology Research Center of Food Non-Thermal Processing, YibinXihua University Research Institute, Yibin 644004, China

1 These authors contributed equally to the work.

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides (OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer (named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.

Keywords

AptamerOrganophosphorus pesticidesRecognition mechanismSequence optimization

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Food Science and Human Wellness
Volume 12 Issue 5,
September 2023
Pages 1708-1715
DOI: 10.1016/j.fshw.2023.02.033
Cite this article:
Chen P, Hu C, Tao X, et al. Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food. Food Science and Human Wellness, 2023, 12(5): 1708-1715. https://doi.org/10.1016/j.fshw.2023.02.033

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Received: 23 July 2021
Revised: 30 August 2021
Accepted: 24 October 2021
Published: 21 March 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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