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Research Article | Open Access

Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells

Lingchao Miaoa, Haolin Zhanga, Meng Sam Cheonga, Ruting Zhonga, Paula Garcia-Oliveirab, Miguel A. Prietob, Ka-Wing Chengc, Mingfu Wangc,d, Hui Caob,e, Shaoping Nief, Jesus Simal-Gandarab, Wai San Cheanga( ), Jianbo Xiaob,g( )
Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense E-32004, Spain
Institute for Advanced Study, Shenzhen University, Shenzhen 508060, China
School of Biological Sciences, The University of Hong Kong, Hong Kong 999077, China
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang 330047, China
International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China

Peer review under responsibility of KeAi Communications Co., Ltd.

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Abstract

Dietary flavonoids are abundant in natural plants and possess multiple pharmacological and nutritional activities. In this study, apigenin, luteolin, and baicalein were chosen to evaluate their anti-diabetic effect in high-glucose and dexamethasone induced insulin-resistant (IR) HepG2 cells. All flavonoids improves the glucose consumption and glycogen synthesis abilities in IR-HepG2 cells via activating glucose transporter protein 4 (GLUT4) and phosphor-glycogen synthase kinase (GSK-3β). These flavonoids significantly inhibited the production of reactive oxygen species (ROS) and advanced glycation end-products (AGEs), which were closely related to the suppression of the phosphorylation form of NF-κB and P65. The expression levels of insulin receptor substrate-1 (IRS-1), insulin receptor substrate-2 (IRS-2) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in IR-HepG2 cells were all partially activated by the flavonoids, with variable effects. Furthermore, the intracellular metabolic conditions of the flavonoids were also evaluated.

Keywords

Reactive oxygen species (ROS)Signaling pathwayLuteolinBaicaleinApigeninInsulin-resistant HepG2 cellsAdvanced glycation end-products (AGEs)Glycogen synthase kinase (GSK-3β)Glucose transporter protein 4 (GLUT4)

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Food Science and Human Wellness
Volume 12 Issue 6,
November 2023
Pages 1991-2000
DOI: 10.1016/j.fshw.2023.03.021
Cite this article:
Miao L, Zhang H, Cheong MS, et al. Anti-diabetic potential of apigenin, luteolin, and baicalein via partially activating PI3K/Akt/Glut-4 signaling pathways in insulin-resistant HepG2 cells. Food Science and Human Wellness, 2023, 12(6): 1991-2000. https://doi.org/10.1016/j.fshw.2023.03.021

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Received: 26 June 2021
Revised: 12 July 2021
Accepted: 22 July 2021
Published: 04 April 2023
© 2023 Beijing Academy of Food Sciences.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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