AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Genes & Diseases Article
PDF (2 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Full Length Article | Open Access

NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue

Boston University, Henry M. Goldman School of Dental Medicine, Department of Endodontics, Boston, MA 02118, USA
United Arab Emirates University, Department of Anatomy, Faculty of Medicine and Health Sciences, Al-Ain, United Arab Emirates
University of Tennessee Health Science Center, College of Dentistry, Department of Bioscience Research, Memphis, TN 38163, USA

Peer review under responsibility of Chongqing Medical University.

d Current address: Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, 875 Union Avenue, Memphis, TN 38163, USA.

Show Author Information

Abstract

NOTCH plays a role in regulating stem cell function and fate decision. It is involved in tooth development and injury repair. Information regarding NOTCH expression in human dental root apical papilla (AP) and its residing stem cells (SCAP) is limited. Here we investigated the expression of NOTCH3, its ligand JAG1, and mesenchymal stem cell markers CD146 and STRO-1 in the AP or in the primary cultures of SCAP isolated from AP. Our in situ immunostaining showed that in the AP NOTCH3 and CD146 were co-expressed and associated with blood vessels having NOTCH3 located more peripherally. In cultured SCAP, NOTCH3 and JAG1 were co-expressed. Flow cytometry analysis showed that 7%, 16% and 98% of the isolated SCAP were positive for NOTCH3, STRO-1 and CD146, respectively with a rare 1.5% subpopulation of SCAP co-expressing all three markers. The expression level of NOTCH3 reduced when SCAP underwent osteogenic differentiation. Our findings are the first step towards defining the regulatory role of NOTCH3 in SCAP fate decision.

Keywords

OsteogenesisStemnessApical papillaCD146JAG1NOTCH3SCAPSTRO-1

References

1

Windley W, Teixeira F, Levin L, Sigurdsson A, Trope M. Disinfection of immature teeth with a triple antibiotic paste. J Endod. 2005;31:439–443.
Crossref Google Scholar
2

Shimizu E, Ricucci D, Albert J, Alobaid AS, Gibbs JL, Huang GT, Lin LM. Clinical, radiographic, and histological observation of a human immature permanent tooth with chronic apical abscess after revitalization treatment. J Endod. 2013;39:1078–1083.
Crossref Google Scholar
3

Iwaya SI, Ikawa M, Kubota M. Revascularization of an immature permanent tooth with apical periodontitis and sinus tract. Dent traumatol. 2001;17:185–187.
Crossref Google Scholar
4

Banchs F, Trope M. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod. 2004;30:196–200.
Crossref Google Scholar
5

Chueh LH, Huang G. Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod. 2006;32:1205–1213.
Crossref Google Scholar
6

Sonoyama W, Liu Y, Fang D, et al. Mesenchymal stem cell-mediated functional tooth regeneration in swine. PloS one. 2006;1:e79.
Crossref Google Scholar
7

Sonoyama W, Liu Y, Yamaza T, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008;34:166–171.
Crossref Google Scholar
8

Huang GT, Sonoyama W, Liu Y, Liu H, Wang S, Shi S. The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod. 2008;34:645–651.
Crossref Google Scholar
9

Huang GTJ, Yamaza T, Shea LD, et al. Stem/progenitor cell–mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng Part A. 2009;16:605–615.
Crossref Google Scholar
10

Kopan R, Ilagan MX. The canonical Notch signaling pathway: unfolding the activation mechanism. Cell. 2009;137:216–233.
Crossref Google Scholar
11

Ghosh S, Paez-Cortez JR, Boppidi K, et al. Activation dynamics and signaling properties of Notch3 receptor in the developing pulmonary artery. J biol Chem. 2011;286:22678–22687.
Crossref Google Scholar
12

Mitsiadis TA, Lardelli M, Lendahl U, Thesleff I. Expression of Notch 1, 2 and 3 is regulated by epithelial-mesenchymal interactions and retinoic acid in the developing mouse tooth and associated with determination of ameloblast cell fate. J cell Biol. 1995;130:407–418.
Crossref Google Scholar
13

Mitsiadis TA, Hirsinger E, Lendahl U, Goridis C. Delta-notch signaling in odontogenesis: correlation with cytodifferentiation and evidence for feedback regulation. Dev Biol. 1998;204:420–431.
Crossref Google Scholar
14

Mitsiadis TA, Regaudiat L, Gridley T. Role of the Notch signalling pathway in tooth morphogenesis. Archives oral Biol. 2005;50:137–140.
Crossref Google Scholar
15

Lovschall H, Mitsiadis TA, Poulsen K, Jensen KH, Kjeldsen AL. Coexpression of Notch3 and Rgs5 in the pericyte-vascular smooth muscle cell axis in response to pulp injury. Int J Dev Biol. 2007;51:715–721.
Crossref Google Scholar
16

Lovschall H, Tummers M, Thesleff I, Füchtbauer E-M, Poulsen K. Activation of the Notch signaling pathway in response to pulp capping of rat molars. Eur J oral Sci. 2005;113:312–317.
Crossref Google Scholar
17

Sun F, Wan M, Xu X, et al. Crosstalk between miR-34a and Notch Signaling Promotes Differentiation in Apical Papilla Stem Cells (SCAPs). J Dent Res. 2014;93:589–595.
Crossref Google Scholar
18

Zhang C, Chang J, Sonoyama W, Shi S, Wang CY. Inhibition of human dental pulp stem cell differentiation by notch signaling. J Dent Res. 2008;87:250–255.
Crossref Google Scholar
19

Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 2003;18:696–704.
Crossref Google Scholar
20

Yang X, van den Dolder J, Walboomers XF, et al. The odontogenic potential of STRO-1 sorted rat dental pulp stem cells in vitro. J tissue Eng Regen Med. 2007;1:66–73.
Crossref Google Scholar
21

Bakopoulou A, Leyhausen G, Volk J, Koidis P, Geurtsen W. Comparative characterization of STRO-1(neg)/CD146(pos) and STRO-1(pos)/CD146(pos) apical papilla stem cells enriched with flow cytometry. Archives oral Biol. 2013;58:1556–1568.
Crossref Google Scholar
22

Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000;97:13625–13630.
Crossref Google Scholar
23

Crisan M, Yap S, Casteilla L, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell stem Cell. 2008;3:301–313.
Crossref Google Scholar
24

Coumailleau F, Furthauer M, Knoblich JA, Gonzalez-Gaitan M. Directional Delta and Notch trafficking in Sara endosomes during asymmetric cell division. Nature. 2009;458:1051–1055.
Crossref Google Scholar
Genes & Diseases
Volume 2 Issue 3,
September 2015
Pages 261-267
DOI: 10.1016/j.gendis.2015.05.003
Cite this article:
Jamal M, Chogle SM, Karam SM, et al. NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue. Genes & Diseases, 2015, 2(3): 261-267. https://doi.org/10.1016/j.gendis.2015.05.003

350

Views

2

Downloads

11

Crossref

N/A

Web of Science

10

Scopus

0

CSCD

Altmetrics
Received: 12 March 2015
Accepted: 18 May 2015
Published: 30 May 2015
© 2015, Chongqing Medical University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Return