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Full Length Article | Open Access

Association of vitamin D receptor gene polymorphism (VDR) with vitamin D deficiency, metabolic and inflammatory markers in Egyptian obese women

Moushira Zakia,( ), Sanaa Kamala, Walaa A. Bashaa, Eman Younessb, Wafaa Ezzatc, Hala El-Bassyounid, Khalda Amre
Biological Anthropology Department, National Research Centre, Giza, Egypt
Medical Biochemistry Department, National Research Centre, Giza, Egypt
Internal Medicine Department, National Research Centre, Giza, Egypt
Clinical Genetics Department, National Research Centre, Cairo, Egypt
Medical Molecular Genetics Department, National Research Centre, Egypt

Peer review under responsibility of Chongqing Medical University.

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Abstract

Vitamin D deficiency might contribute to the pathogenesis of metabolic syndrome and could cause immune disturbance. The aim of this study is to analyze the associations between Vitamin D receptor (VDR) gene polymorphism, serum 25-hydroxy vitamin D, metabolic and inflammatory biomarkers in Egyptian obese women. The study included 201 obese women with vitamin D deficiency and 249 obese matched age healthy controls with sufficient vitamin D levels. Their age ranged between 25 and 35 years. Inflammatory biomarkers (interleukin-6 and C-reactive protein) and serum 25(OH) D were measured by enzyme-linked immunosorbent assay. Insulin resistance (IR) was determined by the homeostasis model assessment of insulin resistance (HOMA-IR).Vitamin D receptor (VDR) gene polymorphisms of FokI, ApaI, and TaqI were studied by PCR using the restriction fragment length polymorphism (RFLP) technique. Obese women with vitamin D deficiency had significant higher values of inflammatory and metabolic parameters compared to controls. Multivariable-logistic regression showed associations between 25(OH) D deficiency and metabolic components when comparing cases with controls. Moreover, cases carrying polymorphic alleles showed significant lower levels of serum 25(OH) D and higher HOMA-IR, blood pressure levels and lipid parameters compared to those with the wild type homozygote in obese cases with vitamin D deficiency. Vitamin D deficiency in Egyptian obese women with vitamin D deficiency is associated with abnormal metabolic components and abnormal inflammatory biomarkers. Moreover, VDR polymorphisms play important role in immune and inflammation status.

Keywords

Inflammatory biomarkersObese womenVitamin D deficiencyVitamin D receptor (VDR) gene polymorphismEgyptian

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Genes & Diseases
Volume 4 Issue 3,
September 2017
Pages 176-182
DOI: 10.1016/j.gendis.2017.07.002
Cite this article:
Zaki M, Kamal S, Basha WA, et al. Association of vitamin D receptor gene polymorphism (VDR) with vitamin D deficiency, metabolic and inflammatory markers in Egyptian obese women. Genes & Diseases, 2017, 4(3): 176-182. https://doi.org/10.1016/j.gendis.2017.07.002

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Received: 29 June 2017
Accepted: 11 July 2017
Published: 18 July 2017
© 2017, Chongqing Medical University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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